Surface DEAE Groups Facilitate Protein Transport on Polymer Chains in DEAE-Modified-and-DEAE-Dextran-Grafted Resins
Surface DEAE Groups Facilitate Protein Transport on Polymer Chains in DEAE-Modified-and-DEAE-Dextran-Grafted Resins
复制标题
表面 DEAE 基团促进 DEAE 改性和 DEAE 葡聚糖接枝树脂中聚合物链上的蛋白质运输
DOI：
10.1002/aic.15412
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Sun Yan
中科院分区：
文献类型：
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作者：
Yu Linling;Gong Lingli;Bai Shu;Sun Yan
用二乙基氨基乙基葡聚糖（DEAE -葡聚糖，DexD）和/或DEAE (D)对Sepharose FF进行改性，制备了三种类型的离子交换剂FF - DexD（接枝-配体树脂）、FF - D（表面-配体树脂）和FF - D - DexD（混合-配体树脂），用于蛋白质吸附平衡和动力学研究。结果表明，接枝DEAE -葡聚糖可显著提高其吸附能力和吸收率（有效扩散率De）。值得注意的是，FF‐DexD和FF‐D‐DexD的devalue值（De/D0> 1.4）比FF‐D的devalue值（De/D0< 0.3）大6倍。更重要的是，表面配体密度的增加大大增强了FF - D - DexD的摄取动力学。结果表明，在混合配体树脂中，表面配体有助于结合蛋白在聚合物链上的运输。也就是说，表面配体作为两个相邻链之间的“转运站”，导致链上结合蛋白的运输增强。该研究揭示了表面配体在促进聚合物接枝离子交换剂上蛋白质摄取动力学中的独特作用。©2016美国化学工程学会化学工程学报，62:3812 - 3819,2016
Sepharose FF was modified with diethylaminoethyl‐dextran (DEAE‐dextran, DexD) and/or DEAE (D) to fabricate three types of ion exchangers FF‐DexD (grafting‐ligand resin), FF‐D (surface‐ligand resin), and FF‐D‐DexD (mixed‐ligand resin), for protein adsorption equilibria and kinetics study. It was found that both adsorption capacity and uptake rate (effective diffusivity, De) were significantly enhanced by grafting DEAE‐dextran. Notably, the Devalues on FF‐DexD and FF‐D‐DexD (De/D0> 1.4) were six times greater than those on FF‐D (De/D0< 0.3). More importantly, the increase of surface‐ligand density greatly enhanced uptake kinetics on FF‐D‐DexD. The results indicate that the surface ligands assisted the transport of bound proteins on polymer chains in the mixed‐ligand resins. That is, surface ligands worked as “transfer stations” between two neighboring chains, resulting in enhanced transport of bound proteins on chains. The research thus disclosed the unique role of surface ligands in facilitating protein uptake kinetics onto polymer‐grafted ion‐exchangers. © 2016 American Institute of Chemical EngineersAIChE J, 62: 3812–3819, 2016