Identification of IgE-binding egg white proteins: comparison of results obtained by different methods.
Identification of IgE-binding egg white proteins: comparison of results obtained by different methods.
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IgE 结合蛋清蛋白的鉴定：不同方法获得的结果的比较。
DOI：
10.1159/000237231
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发表时间：
1996
影响因子：
2.8
通讯作者：
V. Barkholt
中科院分区：
文献类型：
--
作者：
B. Aabin;L. Poulsen;K. Ebbehøj;A. Norgaard;H. Frøkiœr;C. Bindslev‐Jensen;V. Barkholt
研究了 34 份来自有阳性病史和/或卵 RAST 阳性成人血清的 IgE 与卵清蛋白的结合，并研究了常用方法中实验条件对 IgE 结合的影响。还原和未还原蛋清提取物的 SDS-PAGE 后的放射免疫印迹显示出复杂的反应模式。结果通过交叉放射免疫电泳（CRIE）得到证实。放射点免疫结合用于研究过敏原处理对 SDS-PAGE 的效果并评估其他方法。总之，建议结合使用至少两种方法来鉴定 IgE 结合蛋清蛋白。在 34 份血清中，18 份与卵转铁蛋白反应，13 份与卵类粘蛋白反应，11 份与卵清蛋白反应，5 份与溶菌酶反应。与卵清蛋白和溶菌酶结合的 IgE 量通常低于与卵转铁蛋白和卵类粘蛋白结合的量。
The binding of IgE to egg white proteins was investigated for 34 sera from adults with a positive case history and/or positive RAST towards egg, and the impact of experimental conditions on IgE binding in commonly used methods was studied. Radioimmunoblotting after SDS-PAGE of both reduced and unreduced egg white extracts showed complex reaction patterns. The results were confirmed by crossed radioimmunoelectrophoresis (CRIE). Radio dot immunobinding was used to investigate the effect of treatment of allergens for SDS-PAGE and to evaluate the other methods. As a conclusion, the use of combinations of at least two methods is recommended for the identification of IgE-binding egg white proteins. Of the 34 sera, 18 reacted with ovotransferrin, 13 with ovomucoid, 11 with ovalbumin and 5 with lysozyme. The amounts of IgE bound to ovalbumin and lysozyme were generally lower than the amounts bound to ovotransferrin and ovomucoid.