Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis.
Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis.
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DOI：
10.1016/j.gene.2013.11.099
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发表时间：
2014-02
期刊：
影响因子：
3.5
通讯作者：
Yuanli Wang;Qing Li;Jing Xie;Ming Zhu;Wen-juan Sun;Lin He;Qun Wang
中科院分区：
文献类型：
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作者：
Yuanli Wang;Qing Li;Jing Xie;Ming Zhu;Wen-juan Sun;Lin He;Qun Wang
卡尔林环指连接酶(CRL)参与泛素介导的细胞周期调节因子的降解，在配子发生中发挥重要作用。Cullin 4(CUL4)是一类新的泛素E3连接酶的保守核心成分，通过泛素-蛋白酶体途径参与多种调节蛋白的蛋白分解。哺乳动物编码两个CUL4基因，CUL4A和CUL4B，这两个基因在功能上是冗余的。然而，果蝇或其他后生动物只含有一个Cul4基因。在此，我们克隆了中华绒螯蟹CUL4基因，证实中华绒螯蟹中只有一个CUL4蛋白，全长为2777个核苷酸，开放阅读框为2373个核苷酸，编码790个氨基酸残基。实时定量聚合酶链式反应显示，Cul4mRNAs的表达水平在睾丸发育过程中变化很大，最高的转录水平出现在早期阶段。抗CUL4A/4B抗体在生殖系统的定位分析表明，EsCUL4主要分布在精原细胞和初级精母细胞中，在精子发育和成熟过程中逐渐减少。结果表明，单个CUL4蛋白可能在生精过程中起作用。中华绒毛。
The Cullin-RING finger ligases (CRLs) are involved in the ubiquitin-mediated degradation of cell cycle regulators and play an important role in gametogenesis. Cullin 4 (CUL4) is a conserved core component of a new class of ubiquitin E3 ligase, and participates in the proteolysis of several regulatory proteins through the ubiquitin–proteasome pathway. The mammals encode two paralogs of CUL4, CUL4A and CUL4B, and the twoCul4genes are functionally redundant. However, Drosophila or other metazoans only contain oneCul4gene. Here we cloned theCul4gene and confirmed that there is only one protein of CUL4 in Eriocheir sinensis, a full lengthCul4comprised of 2777 nucleotides, an open-reading frame of 2373 bp encoding 790 amino acid residues. The expression level ofCul4mRNAs, as demonstrated by quantitative real-time PCR, varied significantly during testis development, with the greatest transcript levels found at an early stage. Localization analysis using antibodies against CUL4A/4B in the reproductive system showed thatEsCUL4 mainly distribute in spermatogonia and primary spermatocytes, and gradually reduced during the development and maturation of sperm. The results indicated that a single CUL4 protein may play a role in spermatogenesis inE. sinensis.