High content screening for modulators of cardiovascular or global developmental pathways in zebrafish.
High content screening for modulators of cardiovascular or global developmental pathways in zebrafish.
复制标题
高内涵筛选斑马鱼心血管或整体发育途径的调节剂。
DOI：
10.1007/978-1-4939-2269-7_13
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Hong,CharlesC
中科院分区：
文献类型：
--
作者：
Williams,CharlesH;Hong,CharlesC
主要的发育途径在广泛的人类病理学中起着关键作用。化学基因组筛选允许发现新的工具，不仅靶向已知的途径相互作用，而且发现新的，化学上易处理的已知途径的目标。斑马鱼已经成为发育生物学的一个有用的模型，并已得到很好的表征。斑马鱼代表了全能细胞的哈代聚集体，其在内源性背景下并行地大量且同时地评估所有重要途径。这代表了生物测定的金标准，化学靶向选择途径而不引起多效性效应。在这里，我们描述了用于开发高含量筛选试验的方法，实施转基因斑马鱼，以评估表型的变化，已经确定了几类新的化合物，影响发育途径。
Major developmental pathways play critical roles in wide array of human pathologies. Chemical genomic screening allows for the discovery of novel tools not only to target known pathway interactors but also to discover new, chemically tractable targets for known pathways. The zebrafish has emerged as a useful model for developmental biology and has been well characterized. The zebrafish represents a hardy conglomerate of totipotent cells that are massively and simultaneously assessing all significant pathways in parallel in an endogenous context. This represents a gold standard for biological assays, chemically targeting select pathways without causing pleiotropic effects. Here, we describe methods used to develop high content screening assays implementing transgenic zebrafish to assess phenotypic changes that have identified several classes of novel compounds that effect developmental pathways.