Direct visualization and characterization of erythrocyte flow in human retinal capillaries.
Direct visualization and characterization of erythrocyte flow in human retinal capillaries.
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DOI：
10.1364/boe.3.003264
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发表时间：
2012-12-01
影响因子：
3.4
通讯作者：
Metha A
中科院分区：
文献类型：
--
作者：
Bedggood P;Metha A
视网膜脉管系统成像提供了全身血管健康的替代视图，允许对血管病理学进行无创和纵向评估。血管疾病最早的异常现象出现在微脉管系统中，然而目前的成像方法缺乏追踪毛细血管水平血流的时空分辨率。我们在此报告新型成像技术，该技术可以对人类视网膜毛细血管中的红细胞流进行直接、无创的光学成像。这是通过使用可实现高空间分辨率 (1.5 μm) 的自适应光学器件、可实现高时间分辨率 (460 fps) 的 sCMOS 相机技术以及可实现最大红细胞对比度的宽带激光器的可调谐波段来实现的。我们的数据序列上的粒子图像测速用于量化流量。我们观察到速度存在明显的时空变化，范围为 0.3 至 3.3 mm/s，在 130 ms 成像期间，给定毛细管的变化高达 4 倍。成像毛细管网络的平均值和标准差随时间显着变化，但它们的比率仍然是一个相对恒定的参数（0.50 ± 0.056）。我们的观察结果与之前使用不太直接的方法的工作一致，验证了其作为研究人类微血管疾病的研究工具。
Imaging the retinal vasculature offers a surrogate view of systemic vascular health, allowing noninvasive and longitudinal assessment of vascular pathology. The earliest anomalies in vascular disease arise in the microvasculature, however current imaging methods lack the spatiotemporal resolution to track blood flow at the capillary level. We report here on novel imaging technology that allows direct, noninvasive optical imaging of erythrocyte flow in human retinal capillaries. This was made possible using adaptive optics for high spatial resolution (1.5 μm), sCMOS camera technology for high temporal resolution (460 fps), and tunable wavebands from a broadband laser for maximal erythrocyte contrast. Particle image velocimetry on our data sequences was used to quantify flow. We observed marked spatiotemporal variability in velocity, which ranged from 0.3 to 3.3 mm/s, and changed by up to a factor of 4 in a given capillary during the 130 ms imaging period. Both mean and standard deviation across the imaged capillary network varied markedly with time, yet their ratio remained a relatively constant parameter (0.50 ± 0.056). Our observations concur with previous work using less direct methods, validating this as an investigative tool for the study of microvascular disease in humans.