HOST-PARASITE RELATIONSHIPS IN BRUCELLOSIS .2.. DESTRUCTION OF MACROPHAGE CULTURES BY BRUCELLA OF DIFFERENT VIRULENCE
HOST-PARASITE RELATIONSHIPS IN BRUCELLOSIS .2.. DESTRUCTION OF MACROPHAGE CULTURES BY BRUCELLA OF DIFFERENT VIRULENCE
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DOI：
10.1093/infdis/108.3.333
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发表时间：
1961-01-01
影响因子：
6.4
通讯作者：
KROSS, DJ
中科院分区：
文献类型：
--
作者：
FREEMAN, BA;CIRCO, R;KROSS, DJ
本文报道了布鲁氏菌变异株在组织培养中对豚鼠巨噬细胞的致细胞病变作用的比较研究。研究了9种光滑（S）布鲁氏菌菌株和1至4种来自各S亲本的粗糙（R）变体。结果表明，S在所采用的条件下对感染的宿主细胞几乎没有影响。然而，发现R菌株对感染的宿主细胞具有快速破坏性，如通过分光光度法测量宿主细胞成分释放到培养基中所确定的。在所使用的实验条件下，在组织培养中S和R的生长速率没有显着差异，并且发现巨噬细胞对S和R菌株的吞噬作用没有本质上的不同。这些观察结果，沿着发现R菌株在6小时内对宿主巨噬细胞具有破坏性，倾向于支持R对宿主细胞的破坏不是由于机械破坏的观点。
Comparative studies are reported on the cytopathogenic effect of Brucella variants on guinea pig macrophages in tissue culture. Nine smooth (S) Brucella strains and 1 to 4 rough (R) variants derived from each S parent were studied. The findings indicate that S have little or no effect on infected host cells under the conditions employed. R strains, however, were found to be rapidly destructive to infected host cells as determined by the spectrophotometric measurement of the release of host cell constituents into the medium. No significant differences in the rate of growth of S and R in tissue culture was detected under the experimental conditions used and phagocytosis of S and R strains by the macrophages was found not to be essentially different. These observations, along with the finding that R strains are destructive to host macrophages within 6 hours, tends to support the view that destruction of host cells by R is not due to mechanical disruption.