AUTOCRINE GROWTH-STIMULATION OF A HUMAN T-CELL LYMPHOMA LINE BY INTERLEUKIN-2
AUTOCRINE GROWTH-STIMULATION OF A HUMAN T-CELL LYMPHOMA LINE BY INTERLEUKIN-2
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DOI：
10.1073/pnas.82.20.6932
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
DAUTRYVARSAT, A
中科院分区：
文献类型：
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作者：
DUPREZ, V;LENOIR, G;DAUTRYVARSAT, A
肿瘤细胞产生和响应其自身生长因子（自分泌）的能力可能对其生长具有重要意义。我们描述了一个人肿瘤细胞系调节自分泌的生长因子白细胞介素2（IL-2）。这种T淋巴细胞系IARC 301是在没有任何添加的特异性生长因子的情况下从具有T细胞淋巴瘤的有效细胞中建立的。其组成型表达IL-2的生物功能性高亲和力细胞表面受体，如放射性标记的纯化IL-2和单克隆抗体与IL-2受体的结合所示。此外，它还合成IL-2，IL-2与细胞表面受体结合。针对IL-2或IL-2受体的单克隆抗体阻断IARC 301细胞生长。这些发现表明，该肿瘤细胞系的增殖是由涉及内源性IL-2产生及其与细胞表面受体结合的自分泌途径介导的。
The ability of tumor cells to produce and to respond to their own growth factor (autocrine secretion) may be of importance for their growth. We describe a human tumor cell line regulated by an autocrine secretion of the growth factor interleukin 2 (IL-2). This T-lymphocyte cell line, IARC 301, was established from a potent with a T-cell lymphoma in the absence of any added specific growth factor. It constitutively expresses biologically functional high-affinity cell-surface receptors for IL-2 as shown by the binding of both radiolabeled purified IL-2 and monoclonal antibodies to IL-2 receptors. In addition, it synthesizes IL-2, which is bound to cell surface receptors. Monoclonal antibodies directed against either IL-2 or the IL-2 receptor block IARC 301 cell growth. These findings demonstrate that the proliferation of this tumor cell line is mediated by an autocrine pathway involving endogenous IL-2 production and its binding to cell surface receptors.