Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet
Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet
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DOI：
10.1152/ajpgi.00304.2010
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发表时间：
2011-08-01
影响因子：
4.5
通讯作者：
Einspanier, Ralf
中科院分区：
文献类型：
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作者：
Bondzio, Angelika;Gabler, Christoph;Einspanier, Ralf
[10]杨文辉，李文辉.精料日粮对瘤胃上皮细胞差异表达蛋白质的影响。美国生理学胃肠和肝脏生理学杂志301：G260-G268，2011年。首次发表于2011年5月12日; doi：10.1152/ajpgi.00304.2010.-瘤胃上皮适应饮食的变化，代谢，增殖和通透性的良好协调的变化。为了进一步了解控制日粮效应的分子事件，本研究的目的是评估蛋白质表达模式的瘤胃上皮细胞响应不同的饲养制度。绵羊饲喂浓缩补充饲料长达6周。对照组仅接受干草。蛋白质组差异电泳分析结果表明，饲喂干草和精料后2天，瘤胃上皮细胞中有60个蛋白质表达量显著高于精料组（P < 0.05）。40种蛋白质表达上调，20种蛋白质表达下调。6周后，这种饮食，只有14个蛋白质的差异表达。其中11个蛋白表达上调，3个蛋白表达下调。为了确定蛋白质的饮食变化调制，双向电泳与液相色谱电喷雾电离质谱。所选蛋白质，如酯酶D，膜联蛋白5，过氧化物氧还蛋白6，碳酸酐酶I，肌动蛋白相关蛋白3的差异表达进行了验证，通过免疫印迹和/或mRNA分析。所鉴定的蛋白质主要与细胞应激、代谢和分化相关的功能相关。这些结果表明，新的候选蛋白，可能有助于更好地了解信号通路和机制，介导瘤胃上皮细胞适应高浓度的饮食。
Bondzio A, Gabler C, Badewien-Rentzsch B, Schulze P, Martens H, Einspanier R. Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet. Am J Physiol Gastrointest Liver Physiol 301: G260-G268, 2011. First published May 12, 2011; doi:10.1152/ajpgi.00304.2010.-Ruminal epithelium adapts to dietary change with well-coordinated alterations in metabolism, proliferation, and permeability. To further understand the molecular events controlling diet effects, the aim of this study was to evaluate protein expression patterns of ruminal epithelium in response to various feeding regimes. Sheep were fed with a concentrate-supplemented diet for up to 6 wk. The control group received hay only. Proteome analysis with differential in gel electrophoresis technology revealed that, after 2 days, 60 proteins were significantly modulated in ruminal epithelium in a comparison between hay-fed and concentrate-fed sheep (P < 0.05). Forty proteins were upregulated and 20 proteins were downregulated in response to concentrate diet. After 6 wk of this diet, only 14 proteins were differentially expressed. Among these, 11 proteins were upregulated and 3 downregulated. To identify proteins that were modulated by dietary change, two-dimensional electrophoresis was coupled with liquid chromatography electrospray ionization mass spectrometry. The differential expression of selected proteins, such as esterase D, annexin 5, peroxiredoxin 6, carbonic anhydrase I, and actin-related protein 3, was verified by immunoblotting and/or mRNA analysis. The identified proteins were mainly associated with functions related to cellular stress, metabolism, and differentiation. These results suggest new candidate proteins that may contribute to a better understanding of the signaling pathways and mechanisms that mediate rumen epithelial adaptation to high-concentrate diet.