Immunological evidence that the nonhormone binding component of avian steroid receptors exists in a wide range of tissues and species.
Immunological evidence that the nonhormone binding component of avian steroid receptors exists in a wide range of tissues and species.
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免疫学证据表明禽类类固醇受体的非激素结合成分存在于多种组织和物种中。
DOI：
10.1021/bi00344a042
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Toft,DO
中科院分区：
文献类型：
--
作者：
Riehl,RM;Sullivan,WP;Vroman,BT;Bauer,VJ;Pearson,GR;Toft,DO
一种针对真菌蛋白的单克隆抗体已被用于证明多种脊椎动物组织中钼酸盐稳定类固醇受体的非激素结合成分的存在。我们最近发现了类固醇受体在水生真菌绵霉属ambisexualis性mor-phogenesis的男性是类固醇antheridiol执导。这种受体类似于高等生物的受体，表现出8S，钼酸盐稳定的形式。在小鸡输卵管中，一种分子量为90000的蛋白质先前已被证明与孕酮受体的钼酸盐稳定复合物有关。我们从两性甲中分离到了一个分子量约为88000的类似蛋白，并获得了针对它的杂交瘤衍生的单克隆抗体。该小鼠抗水蛭免疫球蛋白G （IgGi）与鸡输卵管细胞质中90000分子量蛋白交叉反应，用于检测哺乳动物组织中类似的90000分子量蛋白。用抗体孵育组织细胞质，将复合物分离到蛋白A-Sepharose上。然后用凝胶电泳分析树脂结合蛋白。该方法显示，在包括大鼠肝脏、小鼠肝脏和子宫、猪卵巢颗粒细胞、人子宫内膜和HeLa细胞在内的几种哺乳动物组织中存在90000分子量的蛋白质。这些结果表明，这种分子量为90000的蛋白并不是鸡输卵管所特有的，而是存在于多种动物的几种不同组织中。该抗体将为进一步研究这些蛋白的生物学作用提供有用的探针。
A monoclonal antibody to a fungal protein has been used todemonstrate the presence of the nonhormone binding component of molybdate-stabilized steroid receptors in a variety of vertebrate tissues. We recently identified a steroid receptor in the aquatic fungus Achlya ambisexualis where sexual mor-phogenesis of the male is directed by the steroid antheridiol. This receptorresembles receptors of higher organisms in exhibiting an 8S, molybdate-stabilized form. In the chick oviduct, a 90 000 molecular weight protein has previously been shown to be associated with the molybdate-stabilized complex of the progesterone receptor. We have isolated a similar protein of molecular weight about 88 000 from A. ambisexualis and have obtained a hybridomal-derived monoclonal antibody directed against it. This mouse anti-Achlya immunoglobulin G)(IgGi) cross-reacts with the 90000 molecular weight protein in chick oviduct cytosol and was used to detect analogous 90000 molecular weight proteins in mammalian tissues. Tissue cytosols were incubated with antibody, and the complexes were isolated onto protein A-Sepharose. The resin-bound proteins were then analyzed by gel electrophoresis. This procedure revealed the presence of 90000 molecular weight proteins in several mammalian tissues including rat liver, mouse liver and uterus, pig ovarian granulosa cells, human endometrium, and HeLa cells. These results demonstrate that the 90 000 molecular weight protein is not peculiar to the chick oviduct but is present in several different tissues from a variety of animals. This antibody should be a useful probe for further studies on the biological role of these proteins.