Cellular Dynamics of Epithelial Clefting During Branching Morphogenesis of the Mouse Submandibular Gland
Cellular Dynamics of Epithelial Clefting During Branching Morphogenesis of the Mouse Submandibular Gland
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DOI：
10.1002/dvdy.22312
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发表时间：
2010-06-01
影响因子：
2.5
通讯作者：
Yamashina, Shohei
中科院分区：
文献类型：
--
作者：
Kadoya, Yuichi;Yamashina, Shohei
我们用非细胞渗透性荧光示踪剂培养了小鼠的原始下颌下腺（SMG），并使用共焦延时显微镜观察了上皮分支形态发生过程中的细胞行为。我们将单个细胞的运动追踪为影子电影。单个上皮细胞动态但不规则地迁移。上皮裂隙通过摆动而延伸，并在分支过程中将细胞簇分成两个芽。我们检查了用层粘连蛋白肽 A5G77f 处理的 SMG 雏形中裂隙的超微结构，层粘连蛋白肽 A5G77f 可诱导上皮裂隙。在裂缝的深端发现了一个带有微丝核心的短细胞质架。我们认为上皮裂隙涉及裂隙底部细胞的动态运动以及裂隙细胞内架子的形成。架子可能在裂口底部形成一个基质附着点，其核心是驱动裂口伸长的微丝。发育动力学 239:1739-1747, 2010。(C) 2010 Wiley-Liss, Inc.
We cultured the rudimental submandibular gland (SMG) of mice with a non-cell-permeable fluorescent tracer, and observed cell behavior during epithelial branching morphogenesis using confocal time-lapse microscopy. We traced movements of individual cells as shadowgraph movies. Individual epithelial cells migrated dynamically but erratically. The epithelial cleft extended by wiggling and separated a cluster of cells into two buds during branching. We examined the ultrastructure of the clefts in SMG rudiments treated with the laminin peptide A5G77f, which induces epithelial clefting. A short cytoplasmic shelf with a core of microfilaments was found at the deep end of the cleft. We propose that epithelial clefting involves a dynamic movement of cells at the base of the cleft, and the formation of a shelf within a cleft cell. The shelf might form a matrix attachment point at the base of the cleft with a core of microfilaments driving cleft elongation. Developmental Dynamics 239:1739-1747, 2010. (C) 2010 Wiley-Liss, Inc.