Induction of apoptosis in human leukemia K562 cells by cyclic lipopeptide from Bacillus subtilis natto T-2
Induction of apoptosis in human leukemia K562 cells by cyclic lipopeptide from Bacillus subtilis natto T-2
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DOI：
10.1016/j.peptides.2007.06.014
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发表时间：
2007-07-01
期刊：
影响因子：
3
通讯作者：
Liu, F.
中科院分区：
文献类型：
--
作者：
Wang, C. L.;Ng, T. B.;Liu, F.
从枯草芽孢杆菌纳豆 T-2 中纯化的新型环状脂肽 (CLP) 可剂量依赖性地抑制人白血病 K562 细胞的生长。荧光染色结果表明CLP引起K562细胞凋亡。流式细胞术分析还表明，CLP 通过细胞阻滞在 G1 期引起 K562 细胞剂量依赖性凋亡。 Western blotting显示，CLP诱导的K562细胞凋亡与caspase-3和聚（ADP-核糖）聚合酶（PARP）蛋白有关。据估计，CLP 通过诱导细胞凋亡来抑制 K562 细胞的增殖。 (c) 2007 年，爱思唯尔公司出版。
A new cyclic lipopeptide (CLP) purified from Bacillus subtilis natto T-2 dose dependently inhibited growth in human leukemia K562 cells. The results of fluorescent staining indicated that CLP brought about apoptosis in K562 cells. Flow cytometric analysis also demonstrated that CLP caused dose-dependent apoptosis of K562 cells through cell arrest at G1 phase. Western blotting revealed that CLP-induced apoptosis in K562 cells was associated with caspase-3 and poly(ADP-ribose)polymerase (PARP) protein. It is estimated that CLP inhibited proliferation in K562 cells by inducing apoptosis. (c) 2007 Published by Elsevier Inc.