Tyrosine-derived polycarbonate scaffolds for bone regeneration in a rabbit radius critical-size defect model
Tyrosine-derived polycarbonate scaffolds for bone regeneration in a rabbit radius critical-size defect model
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DOI：
10.1088/1748-6041/10/3/035001
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发表时间：
2015-06-01
影响因子：
4
通讯作者：
Hollinger, Jeffrey O.
中科院分区：
文献类型：
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作者：
Kim, Jinku;McBride, Sean;Hollinger, Jeffrey O.
本研究的目的是利用酪氨酸来源的聚碳酸酯支架库中的特定聚合物组合物（E1001(1k)），外加磷酸钙（CP）配方（E1001(1k) + CP）和重组人骨形态发生蛋白-2 (rhBMP-2)，来确定兔桡骨临界尺寸缺陷（CSD）模型的骨再生情况。使用特定剂量的rhBMP-2（0、17和35 μ g/支架）。将E1001(1k) + CP支架植入新西兰大白兔桡骨骨干单侧节段缺损（长度为15mm）。植入后4周和8周，采用显微计算机断层扫描（mu CT）、组织学和组织形态学测定骨再生情况。定量结果数据表明，含有rhBMP-2的E1001(1k) + CP支架具有生物相容性，促进了节段性骨缺损的骨再生。植入部位的组织学检查显示，E1001(1k) + CP制成的支架在长达8周的测试期间没有引起不良的细胞或组织反应。值得注意的是，与单独由E1001(1k) + CP组成的支架相比，将极少量的rhBMP-2掺入支架（低至17 μ g/缺陷位点）可显著促进骨再生。这一发现表明E1001(1k) + CP可能是兔桡骨缺损模型中骨再生的有效平台，只需要最小剂量的rhBMP-2。
The aim of the study was to determine bone regeneration in a rabbit radius critical-size defect (CSD) model using a specific polymer composition (E1001(1k)) from a library of tyrosine-derived polycarbonate scaffolds coated with a calcium phosphate (CP) formulation (E1001(1k) + CP) supplemented with recombinant human bone morphogenetic protein-2 (rhBMP-2). Specific doses of rhBMP-2 (0, 17, and 35 mu g/scaffold) were used. E1001(1k) + CP scaffolds were implanted in unilateral segmental defects (15 mm length) in the radial diaphyses of New Zealand White rabbits. At 4 and 8 weeks post-implantation, bone regeneration was determined using micro-computed tomography (mu CT), histology, and histomorphometry. The quantitative outcome data suggest that E1001(1k) + CP scaffolds with rhBMP-2 were biocompatible and promoted bone regeneration in segmental bone defects. Histological examination of the implant sites showed that scaffolds made of E1001(1k) + CP did not elicit adverse cellular or tissue responses throughout test periods up to 8 weeks. Noteworthy is that the incorporation of a very small amount of rhBMP-2 into the scaffolds (as low as 17 mu g/defect site) promoted significant bone regeneration compared to scaffolds consisting of E1001(1k) + CP alone. This finding indicates that E1001(1k) + CP may be an effective platform for bone regeneration in a critical size rabbit radius segmental defect model, requiring only a minimal dose of rhBMP-2.