The Complete Mitochondrial Genome of Aleurocanthus camelliae: Insights into Gene Arrangement and Genome Organization within the Family Aleyrodidae.
The Complete Mitochondrial Genome of Aleurocanthus camelliae: Insights into Gene Arrangement and Genome Organization within the Family Aleyrodidae.
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DOI：
10.3390/ijms17111843
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发表时间：
2016-11-07
影响因子：
5.6
通讯作者：
Peng P
中科院分区：
文献类型：
--
作者：
Chen SC;Wang XQ;Li PW;Hu X;Wang JJ;Peng P
粉虱科物种的线粒体 (mt) 基因组中存在大量基因重排和转移 RNA 基因缺失。为了了解粉虱科的 mt 基因组是如何进化的，我们对茶粉虱的完整 mt 基因组进行了测序，并对所有报道的粉虱 mt 基因组进行了比较分析。茶树的 mt 基因组长 15,188 bp，由 13 个蛋白质编码基因、2 个 rRNA 基因、21 个 tRNA 基因和一个推定的控制区组成（GenBank：KU761949）。在此基因组中尚未观察到 tRNA 基因 trnI。 mt基因组具有独特的基因顺序，并与金合欢树共享大部分基因边界。 21 个 tRNA 基因中有 19 个具有传统的三叶草形二级结构，其中两个（trnS1 和 trnS2）缺乏二氢尿苷 (DHU) 臂。使用 ARWEN 和同源序列比对，我们鉴定了 5 个 tRNA 基因，并修改了 3 个粉虱 mt 基因组的注释。这一结果表明，大多数缺失基因存在于基因组中，但由于缺乏技术和推理序列而尚未被识别。采用最大似然法和贝叶斯推理方法推断了11种烟粉虱和果蝇之间的系统发育关系。茶粉虱和金合欢粉虱形成姐妹群，所有三种烟粉虱和两种后烟粉虱菌株聚集在一起。这些结果与从基因顺序推断的关系相同。我们推断，基因重排在粉虱进化而来的mt基因组中发挥着重要作用。
There are numerous gene rearrangements and transfer RNA gene absences existing in mitochondrial (mt) genomes of Aleyrodidae species. To understand how mt genomes evolved in the family Aleyrodidae, we have sequenced the complete mt genome of Aleurocanthus camelliae and comparatively analyzed all reported whitefly mt genomes. The mt genome of A. camelliae is 15,188 bp long, and consists of 13 protein-coding genes, two rRNA genes, 21 tRNA genes and a putative control region (GenBank: KU761949). The tRNA gene, trnI, has not been observed in this genome. The mt genome has a unique gene order and shares most gene boundaries with Tetraleurodes acaciae. Nineteen of 21 tRNA genes have the conventional cloverleaf shaped secondary structure and two (trnS1 and trnS2) lack the dihydrouridine (DHU) arm. Using ARWEN and homologous sequence alignment, we have identified five tRNA genes and revised the annotation for three whitefly mt genomes. This result suggests that most absent genes exist in the genomes and have not been identified, due to be lack of technology and inference sequence. The phylogenetic relationships among 11 whiteflies and Drosophila melanogaster were inferred by maximum likelihood and Bayesian inference methods. Aleurocanthus camelliae and T. acaciae form a sister group, and all three Bemisia tabaci and two Bemisia afer strains gather together. These results are identical to the relationships inferred from gene order. We inferred that gene rearrangement plays an important role in the mt genome evolved from whiteflies.