Expression, purification, crystallization and preliminary X-ray analysis of NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver
Expression, purification, crystallization and preliminary X-ray analysis of NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver
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甲状腺切除鸡脂肪肝特异性表达的 NAD(P)H 依赖性羰基还原酶的表达、纯化、结晶和初步 X 射线分析
DOI：
10.1107/s1744309112046453
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Toshihisa Ohshima
中科院分区：
文献类型：
--
作者：
Kazunari Yoneda;Yudai Fukuda;Takeshi Shibata;Tomohiro Araki;Takahiro Nikki;Haruhiko Sakurba;Toshihisa Ohshima
以聚乙二醇300为沉淀剂，采用坐滴气相扩散法对去甲状腺鸡脂肪肝特异表达的NAD（P）H依赖性羰基还原酶进行了结晶。晶体属于单斜晶系空间群C2，晶胞参数a = 104.26，B = 81.32，c = 77.27 μ m，β = 119.43°，在光子工厂的NE3A光束线上衍射至1.86 μ m分辨率。 总体Rmerge为5.4%，数据完整性为99.4%。
An NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 104.26, b = 81.32, c = 77.27 Å, β = 119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%.