The detection of Epstein-Barr virus DNA in lung tissue from patients with idiopathic pulmonary fibrosis
The detection of Epstein-Barr virus DNA in lung tissue from patients with idiopathic pulmonary fibrosis
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DOI：
10.1164/ajrccm.159.4.9807077
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发表时间：
1999-04-01
影响因子：
24.7
通讯作者：
Woodcock, AA
中科院分区：
文献类型：
--
作者：
Stewart, JP;Egan, JJ;Woodcock, AA
特发性肺纤维化（IPF）是一种诱发因素尚不清楚的临床综合征。先前曾使用血清学和免疫组织化学提示 Epstein-Barr 病毒 (EBV) 与 IPF 之间存在关联。本研究旨在确认 IPF 患者肺组织中是否存在 EBV DNA。通过免疫组织化学和聚合酶链反应 (PCR) 分析，对 27 名 IPF 患者和 28 名对照受试者手术获得的肺组织中是否存在 EBV 进行了调查。免疫组织化学使用 EBV 裂解周期抗原（gp340/220 和 VCA）特异性抗体。巢式 PCR 分析使用 EBV 特异性寡核苷酸引物，对 EBV DNA 的一份拷贝敏感。通过免疫组织化学检测，27 名 IPF 患者中有 12 名 (44%) 和 28 名对照受试者中的 3 名 (10%) EBV 呈阳性 (p = 0.005)。通过 PCR 检测，13 名 IPF 患者 (48%) 和 4 名对照受试者 (14%) EBV 呈阳性 (p = 0.007)。其中 11 名：IPF 患者 (41%) 和对照受试者均未通过免疫组织化学和 PCR 检测显示 EBV 阳性 (p = < 0.001)，这些数据进一步表明 EBV 与 IPF 之间存在关联。此外，它还定义了一种检测肺组织中 EBV 的新方法。 EBV可能参与该病的发病机制；然而，还需要进一步研究来确定因果关系。
Idiopathic pulmonary fibrosis (IPF) is a clinical syndrome in which the precipitating factors are unclear. An association between Epstein-Barr Virus (EBV) and IPF had previously been suggested using serology and immunohistochemistry. This study sought confirmation of the presence of EBV DNA in the lung tissue of patients with IPF. Lung tissue obtained surgically from 27 patients with IPF and 28 control subjects was investigated for the presence of EBV by immunohistochemistry and polymerase chain reaction (PCR) analysis. Immunohistochemistry used antibodies specific for EBV lytic cycle antigens (gp340/220 and VCA). Nested PCR analysis used oligonucleotide primers specific for EBV and was sensitive to one copy of EBV DNA. Twelve of the 27 patients with IPF (44%) and three of the 28 control subjects (10%) were EBV positive by immunohistochemistry (p = 0.005). Thirteen of the patients with IPF (48%) and four of the control subjects (14%) were EBV positive by PCR (p = 0.007). Eleven of: the patients with IPF (41%) and none of the control subjects were EBV positive by both immunohistochemistry and PCR (p = < 0.001), These data further suggest an association between EBV and IPF. In addition it defines a novel method for detecting EBV in lung tissue. EBV may be involved in the pathogenesis of the disease; however, further studies are required to establish a causal relationship.