FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain.
FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain.
复制标题
DOI：
--
复制
发表时间：
2002-08
期刊：
影响因子：
4.6
通讯作者：
L. Maves;William R. Jackman;C. Kimmel
中科院分区：
文献类型：
--
作者：
L. Maves;William R. Jackman;C. Kimmel
脊椎动物的后脑分为菱形节是高度保守的，但早期的后脑模式是如何建立的还没有很好的理解。我们发现，菱体4（r4）的功能作为一个早期分化的信号中心在斑马鱼后脑。对斑马鱼后脑发育的时间推移分析表明，r4首先形成，后脑神经元的分化首先发生在r4。两种信号分子，FGF 3和FGF 8，都在r4早期表达，共同需要与r4相邻的菱形体的发育，特别是r5和r6。r4细胞的移植可以诱导r5/r6标志物的表达，也可以诱导FGF 3或FGF 8的错误表达。遗传镶嵌分析也支持FGF信号作用于r4的作用。总之，我们的研究结果表明，FGF介导的菱形间信号在促进早期后脑模式化中起着至关重要的作用，并强调了组织中心在脊椎动物神经板模式化中的重要性。
The segmentation of the vertebrate hindbrain into rhombomeres is highly conserved, but how early hindbrain patterning is established is not well understood. We show that rhombomere 4 (r4) functions as an early-differentiating signaling center in the zebrafish hindbrain. Time-lapse analyses of zebrafish hindbrain development show that r4 forms first and hindbrain neuronal differentiation occurs first in r4. Two signaling molecules, FGF3 and FGF8, which are both expressed early in r4, are together required for the development of rhombomeres adjacent to r4, particularly r5 and r6. Transplantation of r4 cells can induce expression of r5/r6 markers, as can misexpression of either FGF3 or FGF8. Genetic mosaic analyses also support a role for FGF signaling acting from r4. Taken together, our findings demonstrate a crucial role for FGF-mediated inter-rhombomere signaling in promoting early hindbrain patterning and underscore the significance of organizing centers in patterning the vertebrate neural plate.