Antimicrobial resistance of Neisseria gonorrhoeae and high prevalence of ciprofloxacin-resistant isolates in Japan, 1993 to 1998
Antimicrobial resistance of Neisseria gonorrhoeae and high prevalence of ciprofloxacin-resistant isolates in Japan, 1993 to 1998
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DOI：
10.1128/jcm.38.2.521-525.2000
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发表时间：
2000-02-01
影响因子：
9.4
通讯作者：
Naito, S
中科院分区：
文献类型：
--
作者：
Tanaka, M;Nakayama, H;Naito, S
为了评估1993年至1998年在日本分离的淋球菌的耐药性，对502株淋球菌进行了药敏试验。所选分离株的特征在于缺陷型和gyrA和parC基因的喹诺酮耐药决定区（QRDR）内的突变分析，赋予氟喹诺酮耐药的生物体。质粒介导的青霉素耐药性（产青霉素酶淋病奈瑟菌）从1993-1994年（7.9%）到1997-1998年（2.0%）显著下降。染色体介导的青霉素耐药从1993-1994年（12.6%）下降到1995-1996年（1.9%），然后在1997-1998年（10.7%）增加。染色体介导的四环素耐药率从1993-1994年的3.3%下降到1997-1998年的2.0%，未发现质粒介导的高水平四环素耐药，环丙沙星耐药（MIC ≥ 1 μ g/ml）的菌株从1993-1994年的6.6%上升到1997-1998年的24.4%。需要脯氨酸的菌株对环丙沙星的敏感性低于原养型或需要精氨酸的菌株。环丙沙星耐药菌株在GyrA和ParC蛋白的QRDR内含有三个或四个氨基酸取代。
To assess the antimicrobial resistance of Neisseria gonorrhoeae isolated from 1993 through 1998 in Japan, susceptibility testing was conducted on 502 isolates. Selected isolates were characterized by auxotype and analysis for mutations within the quinolone resistance-determining region (QRDR) in the gyrA and parC genes, which confer fluoroquinolone resistance on the organism. Plasmid-mediated penicillin resistance (penicillinase-producing N, gonorrhoeae) decreased significantly from 1993-1994 (7.9%) to 1997-1998 (2.0%). Chromosomally mediated penicillin resistance decreased from 1993-1994 (12.6%) to 1995-1996 (1.9%) and then increased in 1997-1998 (10.7%). Chromosomally mediated tetracycline resistance decreased from 1993-1994 (3.3%) to 1997-1998 (2.0%), and no plasmid-mediated high-level tetracycline resistance was found. Isolates with ciprofloxacin resistance (MIC greater than or equal to 1 mu g/ml) increased significantly from 1993-1994 (6.6%) to 1997-1998 (24.4%). The proline-requiring isolates were less susceptible to ciprofloxacin than the prototrophic or arginine-requiring isolates. Ciprofloxacin-resistant isolates contained three or four amino acid substitutions within the QRDR in the GyrA and ParC proteins.