Activation of cyclin E-dependent kinase by DNA-damage signals during apoptosis.
Activation of cyclin E-dependent kinase by DNA-damage signals during apoptosis.
复制标题
细胞凋亡过程中 DNA 损伤信号激活细胞周期蛋白 E 依赖性激酶。
DOI：
10.1006/bbrc.1995.2353
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Yu,C
中科院分区：
文献类型：
--
作者：
Dou,QP;An,B;Yu,C
将 HL-60 细胞预先暴露于 DNA 损伤剂阿糖胞苷 (Ara-C) 中，显着诱导与细胞周期蛋白 E (CycE-H1K) 相关的 H1 激酶活性水平，但与细胞周期蛋白 A 无关。这种诱导是细胞周期无关的，并伴随细胞活力丧失，这是细胞凋亡的晚期事件。当用低浓度的 Ara-C 处理 HL-60 细胞的 Ara-C 抗性变体时，未观察到 CycE-H1K 和细胞凋亡。这两个事件都是在耐药细胞中诱导的，但只有在用更高浓度的 Ara-C 处理较长时间后才诱导。 DNA 损伤诱导的 CycE-H1K 被认为参与晚期细胞凋亡检查点。
Preexposure of HL-60 cells to a DNA-damaging agent, cytosine arabinoside (Ara-C), dramatically induced the levels of H1 kinase activities associated with cyclin E (CycE-H1K) but not cyclin A. This induction was cell cycle-independent and accompanied by loss of cell viability, a late event in apoptosis. When an Ara-C-resistant variant of HL-60 cells was treated with Ara-C at a low concentration, neither CycE-H1K nor apoptosis were observed. Both events were induced in the resistant cells but only after treatment with Ara-C at a much higher concentration for a longer period. The DNA-damage-induced CycE-H1K is proposed to be involved in a late apoptosis checkpoint.