A Signal Amplification Scheme for Ultrasensitive Amperometric Detection in Flowing Streams
A Signal Amplification Scheme for Ultrasensitive Amperometric Detection in Flowing Streams
复制标题
流动流中超灵敏电流检测的信号放大方案
DOI：
10.1021/ac990477x
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Creager
中科院分区：
文献类型：
--
作者：
P. Radford;Andrew French;S. Creager
本文提出了一种新的超灵敏安培电化学检测方法，用于静态溶液和流动液中氧化还原活性分子的检测。该方法基于通过与溶液中的牺牲电子供体反应来连续再生电化学氧化的分析物。它利用电极上的选择性涂层，选择该涂层以具有允许分析物的相对容易的电氧化但也抑制牺牲电子供体的电氧化的性质。超灵敏检测羟甲基二茂铁（HMFc）作为一个模型分析物，使用亚铁氰化物作为牺牲电子供体证明在十二硫醇涂层的金电极。在10-4和10-7 M之间的浓度范围内的分析物注射的流动注射模式中获得几百到几千的信号放大因子，其中峰可以在有和没有放大的情况下辨别。甚至更高的放大系数估计。
A new signal amplification scheme for ultrasensitive amperometric electrochemical detection of redox-active molecules in quiescent solution and in flowing streams is described. The method is based upon a continuous regeneration of electrochemically oxidized analytes by reaction with a sacrificial electron donor in solution. It utilizes a selective coating on the electrode that is chosen to have properties which allow for relatively facile electrooxidation of analyte but which also inhibit electrooxidation of the sacrificial electron donor. Ultrasensitive detection of hydroxymethylferrocene (HMFc) as a model analyte using ferrocyanide as the sacrificial electron donor is demonstrated at a dodecanethiol-coated gold electrode. Signal amplification factors of several hundred to several thousand are obtained in flow injection mode for analyte injections in a concentration range between 10-4 and 10-7 M where peaks can be discerned both with and without amplification. Even higher amplification factors are estima...