Protective Role of Taurine Against Morphine-Induced Neurotoxicity in C6 Cells via Inhibition of Oxidative Stress
Protective Role of Taurine Against Morphine-Induced Neurotoxicity in C6 Cells via Inhibition of Oxidative Stress
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DOI：
10.1007/s12640-011-9247-x
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发表时间：
2011-05
影响因子：
3.7
通讯作者：
Jiaqing Zhou;Yan Li;Guangyan Yan;Q. Bu;L. Lv;Yanzhu Yang;Jinxuan Zhao;X. Shao;Yi Deng
中科院分区：
文献类型：
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作者：
Jiaqing Zhou;Yan Li;Guangyan Yan;Q. Bu;L. Lv;Yanzhu Yang;Jinxuan Zhao;X. Shao;Yi Deng
本研究旨在探讨牛磺酸（2-氨基乙磺酸）对吗啡诱导的C6细胞神经毒性的保护作用。结果发现，牛磺酸能显著提高吗啡处理C6细胞的活力，显示出对吗啡诱导的神经毒性的神经保护作用。然而，牛磺酸的这种神经保护作用不能被-氨基丁酸（GABA）受体拮抗剂双库兰所阻断。为检测吗啡对C6细胞的氧化损伤，测定C6细胞超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）水平。吗啡处理48 h后，C6细胞SOD、CAT和GPx活性降低。而牛磺酸能有效改善吗啡诱导的氧化损伤。为了评估牛磺酸的抗凋亡作用，我们在吗啡作用48 h后，用流式细胞术检测caspase-3和Bcl-2的表达，发现吗啡可以下调Bcl-2的表达，而牛磺酸可以逆转吗啡诱导的Bcl-2表达的下降。牛磺酸对caspase-3的表达无影响。综上所述，结果表明牛磺酸具有改善吗啡诱导的C6细胞氧化损伤和凋亡的能力，可能是由于其抗氧化活性而不是激活GABA受体。
This study was carried out to investigate the protective role of taurine (2-aminoethanesulphonicacid) against morphine-induced neurotoxicity in C6 cells. It was found that taurine significantly increased the viability of C6 cells treated by morphine, showing the neuroprotective role against morphine-induced neurotoxicity. However, such neuroprotective effect of taurine could not be blocked by bicuculline, an antagonist of gamma-amino butyrate (GABA) receptor. To determine the oxidative damage induced by morphine, the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured in C6 cells. The decreased activities of SOD, CAT, and GPx in C6 cells were observed after morphine treatment for 48 h. However, taurine administration effectively ameliorated morphine-induced oxidative insult. To estimate anti-apoptosis effect of taurine, flow cytometry analysis as well as detection for caspase-3 and Bcl-2 expressions was performed after morphine exposure for 48 h. It was found that Bcl-2 expression was down regulated by morphine, whereas taurine could reverse morphine-induced decrease in Bcl-2 expression. Taurine showed no effect on caspase-3 expression. Collectively, the results show that taurine possesses the capability to ameliorate morphine-induced oxidative insult and apoptosis in C6 cells, probably due to its antioxidant activity rather than activation of GABA receptors.