Thin polymer layers formed by polyelectrolyte multilayer techniques on biological surfaces
Thin polymer layers formed by polyelectrolyte multilayer techniques on biological surfaces
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DOI：
10.1021/la9815749
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发表时间：
1999-08-03
期刊：
影响因子：
3.9
通讯作者：
Hubbell, JA
中科院分区：
文献类型：
--
作者：
Elbert, DL;Herbert, CB;Hubbell, JA
薄聚合物膜上形成的模型的组织表面上使用多层膜技术，以评估使用这种技术的可行性，以建立屏障材料的表面上的组织，以改善术后愈合，或表面上的组织工程植入物。通过用聚阳离子孵育异质表面，然后用聚阴离子孵育异质表面，如通过椭圆偏振法和水接触角测量所证实的，在表面上沉积了多层的水。使用聚电解质聚赖氨酸和藻酸盐，这是能够形成复杂的凝胶在生理pH值，而其他人已经证明了线性增长的膜厚度，与此系统，指数增长，在某些条件下，这可能是非常有用的涂层的异质表面，发现特别有利的性能。用多层技术处理的表面包括明胶、成纤维细胞外基质和纤维状I型胶原。所有测试的表面都是高度异质的，并且在处理前与细胞高度粘附。发现形成的薄聚合物层是相对生物惰性的，并且发现组装体的厚度与生物惰性相关，使得可以防止细胞与下面的蛋白质表面的相互作用。聚合物层的厚度可以通过增加吸附的双层的数量以及通过改变处理和洗涤条件来改变，从而增强复合凝胶的形成。
Thin polymer films were formed on models of tissue surfaces using polyelectrolyte multilayer techniques, to evaluate the feasibility of using such techniques to build barrier materials onto the surfaces of tissues to improve postsurgical healing, or on the surfaces of tissue-engineered implants. By incubating heterogeneous surfaces with a polycation, followed by a polyanion, layers of polyelectrolyte were deposited onto the surfaces, as confirmed by ellipsometry and water contact angle measurement. Particularly favorable properties were found using the polyelectrolytes polylysine and alginate, which are capable of forming complex gels at physiologic pH; whereas others have demonstrated linear growth in film thickness, with this system, exponential growth was observed under certain conditions, which may be very useful in the coating of heterogeneous surfaces. Surfaces that were treated with multilayer techniques included gelatin, fibroblast extracellular matrix, and fibrillar type I collagen. All surfaces tested were highly heterogeneous and highly adhesive to cells before treatment. The formed thin polymer layers were found to be relatively bioinert, and the thicknesses of the assembles were found to be correlated with bioinertness, such that interactions of cells with the underlying proteinaceous surface could be prevented. The thickness of the polymer layers could be changed by increasing the number of bilayers adsorbed and also by changing the treatment and washing conditions so as to enhance the formation of complex gels.