A genome-wide association scan of biological processes involved in oral lichen planus and oral squamous cell carcinoma.
A genome-wide association scan of biological processes involved in oral lichen planus and oral squamous cell carcinoma.
复制标题
口腔扁平苔藓和口腔鳞状细胞癌涉及的生物过程的全基因组关联扫描
DOI：
10.1097/md.0000000000007012
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发表时间：
2017-06
期刊：
影响因子：
1.6
通讯作者：
Ma X
中科院分区：
文献类型：
--
作者：
Yang Q;Xu B;Sun H;Wang X;Zhang J;Yu X;Ma X
背景：在本研究中，探讨了口腔扁平苔藓（OLP）恶性转化为口腔鳞状细胞癌（OSCC）的分子机制。方法：对正常受试者以及 OLP 和 OSCC 患者的长非编码 RNA (lncRNA) 和 mRNA 进行高通量测序。绘制 RNA-seq 读数，组装 lncRNA 和 mRNA 转录本，并估计表达水平。 lncRNA的靶点被预测。最后，对差异表达基因（DEG）和lncRNA靶标进行基因本体（GO）和通路富集分析。结果：生成了高质量的序列数据，OSCC、正常和 OLP 样本的比对率较高。总共，从 OPL 与正常、OSCC 与正常以及 OSCC 与 OPL 中分别获得了 820、656 和 582 个 DEG。总共获得了1721个已知的lncRNA和133个预测的lncRNA和靶标。 OSCC 相关的 DEG 显着富集角化，但 OPL 相关的 DEG 没有显着富集。 OPL 和 OSCC 相关 DEG 丰富了嗅觉转导途径。在所有比较中，DEG 和 lncRNA 靶标丰富了对病毒和病毒致癌的防御反应。在 OPL 与正常比较中，与代谢过程相关的 GO 术语被 lncRNA 靶点丰富了，而在其他 2 个比较中，lncRNA 靶点显着丰富了通过 MHC I 类的抗原加工和呈递。结论：在 OLP 向 OSCC 恶性转化过程中，角化和 MHC I 类抗原加工和呈递被激活。此外，嗅觉转导途径可能对 OSCC 很重要。
Background: In this study, the molecular mechanisms underlying malignant transformation from oral lichen planus (OLP) to oral squamous cell carcinoma (OSCC) were examined. Methods: High-throughput sequencing of long noncoding RNAs (lncRNAs) and mRNAs of normal subjects and patients with OLP and OSCC was conducted. RNA-seq reads were mapped, lncRNA and mRNA transcripts were assembled, and expression levels were estimated. The targets of lncRNAs were predicted. Finally, Gene Ontology (GO) and pathway enrichment analyses of differentially expressed genes (DEGs) and lncRNA targets were performed. Results: High-quality sequence data were generated and the mapping ratios for OSCC, normal, and OLP samples were high. In total, 820, 656, and 582 DEGs were obtained from OPL vs. normal, OSCC vs. normal, and OSCC vs. OPL, respectively. A total of 1721 known lncRNAs and 133 predicted lncRNAs and targets were obtained. Keratinization was significantly enriched by OSCC-related DEGs, but not OPL-related DEGs. The pathway of olfactory transduction was enriched by OPL- and OSCC-related DEGs. Defense response to virus and viral carcinogenesis were enriched by DEGs and lncRNA targets in all comparisons. GO term related to the metabolic process was enriched by lncRNA targets in the OPL vs normal comparison, and antigen processing and presentation via MHC class I was significantly enriched by lncRNA targets in the other 2 comparisons. Conclusion: Keratinization and MHC class I antigen processing and presentation were activated during the malignant transformation from OLP to OSCC. Additionally, the olfactory transduction pathway may be important for OSCC.