Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes
Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes
复制标题
DOI：
10.3389/fimmu.2020.01117
复制
发表时间：
2020-06-03
影响因子：
7.3
通讯作者：
Combadiere, Christophe
中科院分区：
文献类型：
--
作者：
Meghraoui-Kheddar, Aida;Barthelemy, Sandrine;Combadiere, Christophe
在小鼠中，单核细胞(Mo)通常被描述为CX3CR1(低)经典Mo(CMO)和CX3CR1(高)非经典Mo(NCMo)，这是基于CX3CR1(+/EGFP)小鼠的EGFP表达，并与人类CX3CR1的表达类似。虽然这个术语被广泛使用，但它可能不能反映CX3CR1在Mo亚群上的表达。通过对稳态和无菌腹膜炎后血Mo的非监督多参数分析，我们观察到CX3CR1的表达并不区分CMO和NCMo亚群。我们的结果强调，尽管EGFP是区分Mo亚型的可靠报告，但CX3CR1(+/EGFP)小鼠的EGFP水平并不反映CX3CR1表达，该表达是由荧光标记的CX3CL1趋化因子和CX3CR1特异性抗体测量的。总之，作者应该谨慎，不要将小鼠经典和非经典Mo识别为CX3CR1(低)和CX3CR1(高)，而应使用替代标记，如Ly6C和CD43的组合。
In mice, monocytes (Mo) are conventionally described as CX3CR1(low)classical Mo (CMo) and CX3CR1(high)non-classical Mo (NCMo) based on the expression of EGFP in Cx3cr1(+/EGFP)mice and by analogy with human CX3CR1 expression. Although this terminology is widely used, it may not reflect the expression of CX3CR1 on Mo subsets. Using an unsupervised multiparametric analysis of blood Mo in steady state and after sterile peritonitis, we observed that CX3CR1 expression did not discriminate the CMo from the NCMo subsets. Our results highlight that despite being a reliable reporter to discriminate Mo subpopulations, EGFP level in Cx3cr1(+/EGFP)mice does not reflect CX3CR1 expression measured by a fluorescently-labeled CX3CL1 chemokine and a CX3CR1 specific antibody. In conclusion, authors should be cautious not to identify murine classical and non-classical Mo as CX3CR1(low)and CX3CR1(high)but rather use alternative markers such as the combination of Ly6C and CD43.