Microarray analysis of gender- and parasite-specific gene transcription in Strongyloides ratti
Microarray analysis of gender- and parasite-specific gene transcription in Strongyloides ratti
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DOI：
10.1016/j.ijpara.2008.02.004
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发表时间：
2008-09-01
影响因子：
4
通讯作者：
Paterson, Steve
中科院分区：
文献类型：
--
作者：
Evans, Helen;Mello, Luciane V.;Paterson, Steve
寄生线虫繁殖并适应宿主体内生活的分子机制尚不清楚。在本研究中，利用微阵列分析探讨了褐鼠寄生线虫鼠类圆线虫不同阶段和性别之间的差异转录。具体来说，确定了自由生活的雌性和自由生活的雄性之间的性别偏向转录，以及寄生雌性和自由生活的雌性之间的寄生偏向转录。在微阵列上估计的3,688个不同转录本中，743个（20％）表现出>1.4倍（2（0.5））的男性偏向转录，689个（19％）女性偏向转录，418个（11％）寄生偏向转录和305个（8％）自由生活偏向转录。在那些表现出最高水平差异转录的转录本中，在雄性中鉴定出主要精子蛋白的直系同源物，在寄生或自由生活的雌性中鉴定出不同的天冬氨酸蛋白酶直系同源物，以及在寄生雌性中鉴定出 hsp-17 分子伴侣的直系同源物。这 3,688 个转录本被分为 12 个簇，因此生命阶段和生物复制之间的转录模式在簇内转录本之间相似，而簇之间则不同。使用从秀丽隐杆线虫推断出的注释，识别出在一个或多个簇中过度代表的基因本体术语，并表明雌性偏向转录与涉及生殖过程和幼虫发育的基因相关，偏向雄性转录与涉及代谢的基因相关，而自由生活偏向转录与涉及体液调节和对外部刺激反应的基因相关。基因本体与偏向寄生虫的转录之间的关联尚不清楚。目前对鼠鼠的发现为详细探索差异调节分子提供了基础，并可能有助于寻找寄生线虫的新药物或疫苗靶点。 (C) 2008 年澳大利亚寄生虫学协会。由 Elsevier Ltd 出版。保留所有权利。
The molecular mechanisms by which parasitic nematodes reproduce and have adapted to life within a host are unclear. In the present study, microarray analysis was used to explore differential transcription among the different stages and sexes of Strongyloides ratti, a parasitic nematode of brown rats. Specifically, gender-biased transcription between free-living females and free-living males, and parasitic-biased transcription between parasitic females and free-living females was determined. Of the estimated 3,688 distinct transcripts represented on the microarray, 743 (20%) exhibited male-biased transcription of >1.4-fold (2(0.5)), 689 (19%) female-biased transcription, 418 (11%) parasitic-biased transcription and 305 (8%) free-living-biased transcription. Among those transcripts that exhibited the highest levels of differential transcription, an orthologue of major sperm protein was identified in males, distinct aspartic protease orthologues in either parasitic or in free-living females, and orthologues of hsp-17 chaperone in parasitic females. These 3,688 transcripts were separated into 12 clusters, such that the pattern of transcription between life-stages and biological replicates was similar among transcripts within a cluster and dissimilar between clusters. Using annotation inferred from Caenorhabditis elegans, gene ontology terms over-represented in one or more clusters were identified and showed that female-biased transcription was associated with genes involved in reproductive processes and larval development, male-biased transcription was linked to genes involved in metabolism, and free-living-biased transcription related to genes involved in the regulation of body fluids and response to external stimulus. The association of gene ontology with parasite-biased transcription was less clear. The present findings for S. ratti provide a basis for a detailed exploration of differentially regulated molecules and might assist in the search for novel drug or vaccine targets in parasitic nematodes. (C) 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.