Heterologously expressed β-hydroxyl fatty acids from a metagenomic library of a marine sponge
Heterologously expressed β-hydroxyl fatty acids from a metagenomic library of a marine sponge
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来自海绵宏基因组库的异源表达的β-羟基脂肪酸
DOI：
10.1016/j.bmcl.2012.10.082
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
I. Abe
中科院分区：
文献类型：
--
作者：
R. He;T. Wakimoto;Y. Egami;H. Kenmoku;T. Ito;Y. Asakawa;I. Abe
基于日本海绵disdermia calyx宏基因组文库的抑菌功能筛选，获得了3-羟基脂肪酸：3-羟基铝酸、3-羟基月桂酸和3-羟基肉豆蔻酸，并在抗菌克隆pDC113中异源表达。3-羟基铝酸对蜡样芽孢杆菌和白色念珠菌有中等抑菌活性。对插入DNA进行序列分析，发现23个可能的orf，其中大多数与细菌脂肪酸合成酶II和脂质A生物合成酶具有同源性。其他orf可能是参与脂质A生物合成的跨膜蛋白。虽然在我们的实验条件下没有检测到脂质A，但作为脂质A成分的β-羟基脂肪酸在pDC113中产生增强。
Functional screening based on the antibacterial activity of a metagenomic library of the Japanese marine sponge, Discodermia calyx, afforded three β-hydroxyl fatty acids: 3-hydroxypalmitic acid, 3-hydroxylauric acid and 3-hydroxymyristic acid, heterologously expressed in an antibacterial clone, pDC113. 3-Hydroxypalmitic acid showed moderate antibacterial activity against Bacillus cereus and Candida albicans. A sequence analysis of the insert DNA revealed 23 putative ORFs, with most sharing homology to bacterial fatty acid synthase II and lipid A biosynthesis enzymes. The other ORFs were probably transmembrane proteins involved in lipid A biosynthesis. Although lipid A was not detected under our experimental conditions, the production of β-hydroxyl fatty acids as components of lipid A were enhanced in pDC113.