Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in human colon carcinoma cells
Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in human colon carcinoma cells
复制标题
DOI：
10.1016/j.taap.2010.04.012
复制
发表时间：
2010-08-01
影响因子：
3.8
通讯作者：
Meschini, Stefania
中科院分区：
文献类型：
--
作者：
De Berardis, Barbara;Civitelli, Gabriele;Meschini, Stefania
工程纳米颗粒在许多工业和生物医学应用中提供了很大的希望，然而关于胃肠道毒性的信息很少。本研究的目的是评估细胞毒性、氧化应激，ZnO纳米粒子诱导人结肠癌LoVo细胞凋亡及促炎介质释放的研究这些纳米粒子的生物活性与其物理化学特性有关，用生长曲线和水溶性四唑法测定细胞毒性，流式细胞仪测定活性氧产生、细胞谷胱甘肽含量、线粒体膜电位变化和凋亡细胞死亡。采用酶联免疫吸附法检测血清中炎性细胞因子的含量（5 μ g/cm（2）对应于11.5 μ g/ml）作用24 h可诱导LoVo细胞存活率显著降低，H2 O2/OH中心点增加，02（-中心点）和GSH减少，线粒体内膜去极化，细胞凋亡和IL-8释放在处理24小时后，更高剂量已经诱导了约98%的细胞毒性。实验数据显示氧化应激可能是在结肠癌细胞中诱导ZnO纳米颗粒细胞毒性的关键途径。此外，毒理学效应与颗粒理化特性之间关系的研究表明，表面积在细胞毒性中不起主要作用（C）2010 Elsevier Inc保留所有权利
Engineered nanoparticles offer great promise in many industrial and biomedical applications, however little information is available about gastrointestinal toxicity The purpose of this study was to assess the cytotoxicity, oxidative stress, apoptosis and proinflammatory mediator release induced by ZnO nanoparticles on human colon carcinoma LoVo cells The biological activity of these particles was related to their physico-chemical characteristics The physico-chemical characteristics were evaluated by analytical electron microscopy The cytotoxicity was determined by growth curves and water-soluble tetrazolium assay The reactive oxygen species production, cellular glutathione content, changes of mitochondrial membrane potential and apoptosis cell death were quantified by flow cytometry. The inflammatory cytokines were evaluated by enzyme-linked immunoadsorbent assay Treatment with ZnO (5 mu g/cm(2) corresponding to 11 5 mu g/ml) for 24 h induced on LoVo cells a significant decrease of cell viability, H2O2/OH center dot increase, 02(-center dot) and GSH decrease, depolarization of inner mitochondrial membranes, apoptosis and IL-8 release Higher doses induced about 98% of cytotoxicity already after 24 h of treatment The experimental data show that oxidative stress may be a key route in inducing the cytotoxicity of ZnO nanoparticles in colon carcinoma cells Moreover, the study of the relationship between toxicological effects and physico-chemical characteristics of particles suggests that surface area does not play a primary role in the cytotoxicity (C) 2010 Elsevier Inc All rights reserved