Studying viruses using solution X-ray scattering.
Studying viruses using solution X-ray scattering.
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DOI：
10.1007/s12551-020-00617-4
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发表时间：
2020-02-01
影响因子：
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通讯作者：
Raviv, Uri
中科院分区：
文献类型：
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作者：
Khaykelson, Daniel;Raviv, Uri
自从世纪发现病毒是一种小的传染源以来，病毒就引起了人类的兴趣。由于许多病毒会导致人类和农业疾病，因此出于生物学和医学目的对它们进行了严格的研究。病毒具有显著的特性，例如其蛋白质包膜的对称性和自组装，成熟为感染性病毒体，结构稳定性和拆卸。溶液X射线散射可以探测溶液中的结构和反应，空间分辨率低至亚纳米，时间分辨率为毫秒。它探测本体溶液，揭示溶液中颗粒的平均形状和平均质量，可用于研究病毒在其生命周期不同阶段的动力学和热力学。在这里，我们回顾了最近的工作，证明了解决方案的X射线散射的能力，在体外研究病毒的生命周期。
Viruses have been of interest to mankind since their discovery as small infectious agents in the nineteenth century. Because many viruses cause diseases to humans and agriculture, they were rigorously studied for biological and medical purposes. Viruses have remarkable properties such as the symmetry and self-assembly of their protein envelope, maturation into infectious virions, structural stability, and disassembly. Solution X-ray scattering can probe structures and reactions in solutions, down to subnanometer spatial resolution and millisecond temporal resolution. It probes the bulk solution and reveals the average shape and average mass of particles in solution and can be used to study kinetics and thermodynamics of viruses at different stages of their life cycle. Here we review recent work that demonstrates the capabilities of solution X-ray scattering to study in vitro the viral life cycle.