Quantitative profile of estrogen receptor variants/isoforms in Taiwanese women with breast cancer
Quantitative profile of estrogen receptor variants/isoforms in Taiwanese women with breast cancer
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DOI：
10.1016/j.ejso.2006.02.010
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发表时间：
2006-06-01
期刊：
影响因子：
3.8
通讯作者：
Young, K. -C.
中科院分区：
文献类型：
--
作者：
Hsiao, W. -C.;Cho, W. -C.;Young, K. -C.
目的：定量检测台湾地区乳腺癌患者ER变异体/异构体。方法：采用实时定量聚合酶链式反应技术对49例乳腺癌及配对的癌旁正常组织中ERα、ERαE3 Delta、ERαE5 Delta、ERαE7 Delta和ERβ的mRNAs进行定量。结果：癌组织和匹配的正常组织中ERβ显著降低(p<；0.001)，而ERa及其变异体的含量保持不变。有淋巴结转移者ERαE7 Delta/ERα(p=0.030)和ERβ/ERα(p=0.035)比值明显低于无淋巴结转移者。结论：ERα、E7 Delta和ERβ对正常乳腺组织ERα可能有调节作用。(C)2006爱思唯尔有限公司。保留所有权利。
Aim: To quantify ER variants/isoforms for women with breast cancer in Taiwan, an area with remarkably low breast cancer rates, but with an early onset and poor prognosis.Methods: Real-time PCR technology was exploited to quantify mRNAs of ER alpha, ER alpha E3 Delta, ER alpha E5 Delta, ER alpha E7 Delta and ER beta, in 49 breast cancer and paired adjacent normal tissues. Clinical parameters were assembled and tested for assocation with the ER expression.Results: Comparison of cancer and matched normal samples showed significantly decreased ER beta (p < 0.001) in cancer tissues, and constant amounts of ERa and their variants. The results revealed significantly lower ER alpha E7 Delta/ER alpha (p = 0.030) and ER beta/ER alpha (p = 0.035) ratios in patients with lymph node (LN) metastasis than in those without LN metastasis.Conclusion: Our data suggests that ER alpha E7 Delta and ER beta may regulate ER alpha in normal human breast tissue. (C) 2006 Elsevier Ltd. All rights reserved.