Detection of cocaine, norcocaine, and cocaethylene in the meconium of premature neonates.
Detection of cocaine, norcocaine, and cocaethylene in the meconium of premature neonates.
复制标题
早产儿胎便中可卡因、去甲可卡因和可卡乙烯的检测。
DOI：
--
复制
发表时间：
1994
影响因子：
1.6
通讯作者：
A. Dusick
中科院分区：
文献类型：
--
作者：
S. Browne;C. Moore;A. Negrusz;I. Tebbett;R. Covert;A. Dusick
我们的目的是通过测定胎粪分析可卡因及其代谢物的可行性来研究妊娠期间可卡因滥用的方法学，用层析方法替代酶免疫分析技术检测尿液。我们的设计如下：胎粪和尿液取自106名极低出生体重早产儿。采用提取和层析分析方法对可卡因及其代谢物进行胎粪分析，并与尿液标准免疫分析方法进行比较。这项工作是在芝加哥大学医院儿科和伊利诺伊大学芝加哥分校药物动力学系进行的。我们的患者都是极低出生体重的早产儿(平均出生体重1109g；平均胎龄29.1周)。性别平均分为男性和女性。结果测量如下：在一些新生儿的胎粪中检测到两种活性代谢物，去甲卡因和可卡因，但在尿液中没有检测到。新生儿中可卡因暴露的确定影响了婴儿在研究研究中的分配以及对新生儿的心理社会评估和随后的治疗。检测的106份胎粪标本中，有21份(19.8%)检出可卡因(n=19，0.24-0.78 mg/kg)、去甲卡因(n=7，0.10-0.56 mg/kg)、可卡因(n=1，0.12 mg/kg)及其组合。在所有样品中均未检测到苯甲酰ecGonine。在免疫分析的尿样中，只有8份(7.5%)的可卡因代谢物呈阳性。我们的结论是胎粪是一种比尿液更好的样本来确定可卡因在子宫内的暴露。
Our objective was to investigate the methodologic detection of cocaine abuse during pregnancy by determining the viability of meconium analysis for cocaine and its metabolites using chromatographic procedures as an alternative to urine testing using enzyme multiplied immunoassay technique. Our design was as follows: meconium and urine were taken from 106 very low birthweight premature babies. Meconium analysis for cocaine and its metabolites using extraction and chromatographic analysis was compared with the criterion standard immunoassay testing for urine. The work was carried out at The University of Chicago Hospital, Department of Pediatrics and the University of Illinois at Chicago, Department of Pharmacodynamics. Our patients were very low birthweight, premature babies (mean birthweight 1109 g; mean gestational age 29.1 weeks). Gender was evenly divided between male and female. The outcome measures were as follows: two active metabolites, norcocaine and cocaethylene, were detected in the meconium, but not in the urine, of some of the neonates. Determination of cocaine exposure in the newborn influenced assignment of babies in research studies as well as psychosocial evaluation and subsequent treatment of the neonate. Our results were: of the 106 meconium samples analyzed, 21 (19.8%) were positive for cocaine (n = 19, 0.24-0.78 mg/kg), norcocaine (n = 7, 0.10-0.56 mg/kg), cocaethylene (n = 1, 0.12 mg/kg) or combinations thereof. Benzoylecgonine was not detected in any of the samples. Of the urine samples analyzed by immunoassay, only 8 (7.5%) were positive for cocaine metabolites. We conclude that meconium is a better sample than urine for determining cocaine exposure in utero.(ABSTRACT TRUNCATED AT 250 WORDS)