Identification of microRNAs and their corresponding targets involved in the susceptibility interaction of wheat response to Puccinia striiformis f. sp tritici
Identification of microRNAs and their corresponding targets involved in the susceptibility interaction of wheat response to Puccinia striiformis f. sp tritici
复制标题
鉴定参与小麦对条锈菌 f 的敏感性相互作用的 microRNA 及其相应靶标。
DOI：
10.1111/ppl.12407
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发表时间：
2016-05-01
影响因子：
6.4
通讯作者：
Kang, Zhensheng
中科院分区：
文献类型：
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作者：
Feng, Hao;Wang, Ting;Kang, Zhensheng
microRNAs（miRNAs）在植物防御反应中起着非常重要的作用。然而，它们在小麦与条锈菌的感病互作中的作用却知之甚少。小麦种（Pst）本研究以杏资9104为材料，分别用Pst强毒小种CYR 32和无菌水接种，构建了两个miRNA文库。共获得1316个候选miRNA，包括从98个pre-miRNA产生的173个已知miRNA。剩下的1143个候选miRNA包括145个保守的和998个小麦特异性miRNA，它们分别由87个和1088个前体miRNA产生。173个已知的和145个保守的miRNA被细分为63个miRNA家族。利用degradome测序技术对小麦miRNAs的靶基因进行了确认。注释的靶基因大多与信号转导或能量代谢有关。此外，我们发现miRNAs与其靶基因形成了复杂的调控网络。通过实时定量PCR（qRT-PCR）进一步分析miRNAs及其靶基因的表达谱，结果表明部分miRNAs参与了小麦与Pst的亲和感病互作。重要的是，tae-miR 1432在小麦受到CYR 32攻击时高度表达，并且预测为钙离子结合蛋白的相应靶基因也表现出上调表达，但表达趋势不同。PC-3 P-7484是一种特异的小麦miRNA，在小麦对Pst感染的应答中高表达，而相应的靶基因ubiquillin的表达显著下调。这些数据为评价miRNAs在小麦-Pst感病互作中的重要调控作用提供了基础。
MicroRNAs (miRNAs) play very important roles in plant defense responses. However, little is known about their roles in the susceptibility interaction between wheat and Puccinia striiformis f. sp. tritici (Pst). In this study, two miRNA libraries were constructed from the leaves of the cultivar Xingzi 9104 inoculated with the virulent Pst race CYR32 and sterile water, respectively. A total of 1316 miRNA candidates, including 173 known miRNAs that were generated from 98 pre-miRNAs, were obtained. The remaining 1143 miRNA candidates included 145 conserved and 998 wheat-specific miRNAs that were generated from 87 and 1088 pre-miRNAs, respectively. The 173 known and 145 conserved miRNAs were sub-classified into 63 miRNA families. The target genes of wheat miRNAs were also confirmed using degradome sequencing technology. Most of the annotated target genes were related to signal transduction or energy metabolism. Additionally, we found that miRNAs and their target genes form complicated regulation networks. The expression profiles of miRNAs and their corresponding target genes were further analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), and the results indicate that some miRNAs are involved in the compatible wheat-Pst susceptibility interaction. Importantly, tae-miR1432 was highly expressed when wheat was challenged with CYR32, and the corresponding target gene, predicted to be a calcium ion-binding protein, also exhibited upregulated expression but a divergent expression trend. PC-3P-7484, a specific wheat miRNA, was highly expressed in the wheat response to Pst infection, while the expression of the corresponding target gene ubiquillin was dramatically downregulated. These data provide the foundation for evaluating the important regulatory roles of miRNAs in wheat-Pst susceptibility interaction.