Methylomonas rhizoryzae sp. nov., a type I methanotroph isolated from the rhizosphere soil of rice.
Methylomonas rhizoryzae sp. nov., a type I methanotroph isolated from the rhizosphere soil of rice.
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根茎甲基单胞菌 sp.
DOI：
10.1007/s10482-020-01487-2
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yan Xin
中科院分区：
文献类型：
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作者：
Zhu Pingping;Cheng Minggen;Pei Dongmei;Liu Yongchuang;Yan Xin
从南京地区水稻根际土壤中分离到一株γ-蛋白细菌GJ 1 T。这些细胞是革兰氏阴性，能动杆与一个单一的极性鞭毛，它们包含I型胞质膜。细胞形成粉红色集落。该菌株同时具有颗粒型甲烷单加氧酶（pMMO）和可溶性甲烷单加氧酶（sMMO），还含有编码分歧型甲烷单加氧酶（pXMO）的pxmABC和编码二氮还原酶的nifH。菌株GJ 1 T能利用甲烷和甲醇作为唯一的碳源，不能利用乙酸、丙酮酸、琥珀酸、柠檬酸、苹果酸、葡萄糖、尿素、甲胺、乙醇和甲酸等其他碳源。细胞在25-33 °C（范围16-37 °C）、pH 6.0-8.0（范围5.5-8.5）和0- 1.2%NaCl（在1.5%NaCl以上无生长）下生长最佳。基于16 S rRNA基因、pmoA和nifH的系统发育分析表明，该分离物属于γ-变形菌纲甲基球菌科甲基单胞菌属。主要的醌被确定为MQ-8，并且观察到主要的脂肪酸为C16：1和C14：0。GJ 1 T的基因组大小约为4.55Mb，DNA G + C含量为53.67mol%，与目前报道的甲基单胞菌属的DNA G + C含量（47- 58mol%）一致。甲基单胞菌属中GJ 1 T株与高山甲基单胞菌Fw 12 E-YT株的平均核苷酸同源性（ANI）和数字DNA-DNA杂交（dDDH）值最高，分别为74.66%和21.40%。因此，表型特征和系统发育分析的结果支持菌株GJ 1 T作为一个新的物种在属甲基单胞菌，即，甲基单胞菌rhizoryzaesp。11月模式菌株为GJ 1 T（= ACCC 61706）。
A gammaproteobacterial methanotroph, strain GJ1T, was isolated from a rhizosphere soil sample of rice in Nanjing, China. The cells were Gram-negative, motile rods with a single polar flagellum, and they contained type I intracytoplasmic membranes. The cells formed pink colonies. The strain possessed both the particulate methane monooxygenase enzyme (pMMO) and the soluble methane monooxygenase enzyme (sMMO).pxmABC, encoding a divergent methane monooxygenase (pXMO), andnifH, which encodes dinitrogenase reductase, were also present. Methane and methanol were utilized as sole carbon sources, while other carbon sources, including acetate, pyruvate, succinate, citrate, malate, glucose, urea, methylamine, ethanol and formate, could not be utilized by strain GJ1T. Cell grew optimally at 25–33 °C (range 16–37 °C), pH 6.0–8.0 (range 5.5–8.5) and 0–1.2% NaCl (no growth above 1.5% NaCl). Phylogenetic analyses based on the 16S rRNA gene,pmoAandnifHshowed that the isolate belongs to the genusMethylomonasof the familyMethylococcaceaewithin the classGammaproteobacteria. The major quinone was determined to be MQ-8, and the major fatty acids were observed to be C16:1and C14:0. The genome size of strain GJ1Tis about 4.55 Mb, and the DNA G + C content of the strain was determined to be 53.67 mol% within the range of the genusMethylomonas(47–58 mol%) reported at present. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain GJ1TandMethylomonas koyamaeFw12E-YTamong the genusMethylomonaswere the highest, and they were only 74.66% and 21.40%, respectively. In consequence, results of phenotypic characterization and phylogenetic analyses support strain GJ1Tas a novel species within the genusMethylomonas, namely,Methylomonas rhizoryzaesp. nov.. The type strain is GJ1T(= ACCC 61706).