Comparative susceptibility of resident and transient hand bacteria to para-chloro-meta-xylenol and triclosan
Comparative susceptibility of resident and transient hand bacteria to para-chloro-meta-xylenol and triclosan
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DOI：
10.1046/j.1365-2672.2002.01691.x
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Handley, PS
中科院分区：
文献类型：
--
作者：
Johnson, SA;Goddard, PA;Handley, PS
目的：确定常驻和暂存皮肤细菌的浮游和生物膜生长菌株对液体洗手皂杀菌剂对氯间二甲酚 (PCMX) 和三氯生的敏感性。方法和结果：通过部分 16S rRNA 基因测序鉴定新鲜分离的手部细菌。选择两种常驻菌株和三种瞬时菌株，以及四种外源潜在瞬时菌株进行杀菌剂敏感性测试。测定浮游细胞的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。常驻菌株和瞬时菌株对两种杀菌剂表现出一系列敏感性（PCMX，MIC 12.5-200 mg l(-1)，MBC 100-400 mg l(-1)；三氯生，MIC 0.6->40 mg l(-1)，MBC 1.3->40 mg l(-1)）。将菌株在 96 孔微量滴定板中附着在聚苯乙烯板上 65 小时，并用杀菌剂激发以确定生物膜抑制浓度和生物膜根除浓度。对于所有测试的菌株，生物膜对 PCMX 的敏感性比浮游细胞低两到八倍。结论：在手上检测到的瞬变非常少。瞬态人群对杀菌剂的敏感性并不比居民更敏感，而且对 PCMX 和三氯生的敏感性取决于菌株。与浮游细胞相比，生物膜生长的菌株对 PCMX 的敏感性较低。 该研究的意义和影响：该研究提供了有关皮肤细菌对典型液体抗菌洗手皂中存在的杀菌剂敏感性的更多知识，并表明此类产品中使用的杀菌剂浓度超过了杀死皮肤上通常存在的少量暂时性细菌所需的浓度。
Aims: To determine the susceptibility of planktonic and biofilm-grown strains of resident and transient skin bacteria to the liquid hand soap biocides para-chloro-meta-xylenol (PCMX) and triclosan.Methods and Results: Freshly isolated hand bacteria were identified by partial 16S rRNA gene sequencing. Two resident and three transient strains, as well as four exogenous potential transient strains, were selected for biocide susceptibility testing. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of planktonic cells were determined. Resident and transient strains showed a range of susceptibilities to both biocides (PCMX, MIC 12.5-200 mg l(-1) , MBC 100-400 mg l(-1) ; triclosan, MIC 0.6- > 40 mg l(-1) , MBC 1.3- > 40 mg l(-1) ). Strains were attached to polystyrene plates for 65 h in 96-well microtitre plates and challenged with biocide to determine the biofilm inhibitory concentration and biofilm eradicating concentration. For all strains tested, biofilms were two- to eightfold less susceptible than planktonic cells to PCMX.Conclusions: Very few transients were detected on the hand. Transients were not more sensitive than residents to the biocides and susceptibility to PCMX and triclosan was strain dependent. Biofilm-grown strains were less susceptible to PCMX than planktonic cells.Significance and Impact of the Study: The study provides increased knowledge about the susceptibility of skin bacteria to biocides present in typical liquid antibacterial hand soaps and suggests that the concentration of biocide employed in such products is in excess of that required to kill the low numbers of transient bacteria typically found on skin.