The Agrobacterium tumefaciens virC1 gene product binds to overdrive, a T-DNA transfer enhancer.
The Agrobacterium tumefaciens virC1 gene product binds to overdrive, a T-DNA transfer enhancer.
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根癌农杆菌 virC1 基因产物与 T-DNA 转移增强子 Overdrive 结合。
DOI：
10.1128/jb.171.12.6845-6849.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
Nester,EW
中科院分区：
文献类型：
--
作者：
Toro,N;Datta,A;Carmi,OA;Young,C;Prusti,RK;Nester,EW
在根癌农杆菌中，邻近T-DNA右边界的顺式活性24个碱基对序列（称为cis-active 24-base-pair sequence）通过增加T-DNA加工水平刺激肿瘤形成。我们实验室的最新结果表明，virC操纵子增强T-DNA加工可能是因为VirC 1蛋白质与DNA相互作用。Toro，A.达塔，M. Yanofsky和E. W. Nester，Proc. Natl. Acad. Sci. USA 85：8558-8562，1988）。我们在这里报告的VirC 1蛋白质的纯化从大肠杆菌的细胞窝藏一个质粒含有的virC基因座的章鱼碱Ti质粒的编码序列。通过凝胶迁移率改变和DNA酶I足迹分析，我们表明，这种纯化的virC 1基因产物结合到T-DNA的右边缘，但不结合到T-DNA的右边缘。
In Agrobacterium tumefaciens, a cis-active 24-base-pair sequence adjacent to the right border of the T-DNA, called overdrive, stimulates tumor formation by increasing the level of T-DNA processing. Recent results from our laboratory have suggested that the virC operon which enhances T-DNA processing probably does so because the VirC1 protein interacts with overdrive (N. Toro, A. Datta, M. Yanofsky, and E. W. Nester, Proc. Natl. Acad. Sci. USA 85:8558-8562, 1988). We report here the purification of the VirC1 protein from cells of Escherichia coli harboring a plasmid containing the coding sequences of the virC locus of the octopine Ti plasmid. By gel mobility shift and DNase I footprinting assays, we showed that this purified virC1 gene product binds to overdrive but not to the right border of T-DNA.