2,4-dinitrobenzenesulfonyl fluoresceins as fluorescent alternatives to Ellman's reagent in thiol-quantification enzyme assays
2,4-dinitrobenzenesulfonyl fluoresceins as fluorescent alternatives to Ellman's reagent in thiol-quantification enzyme assays
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DOI：
10.1002/anie.200500114
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发表时间：
2005-01-01
影响因子：
16.6
通讯作者：
Itoh, N
中科院分区：
文献类型：
--
作者：
Maeda, H;Matsuno, H;Itoh, N
安吉街2923号国际化学版2005，44，2922-2925 www. angewandte。org 2005 Wiley-VCH Verlag GmbH & Co. KGaA，魏因海姆，用5在96孔微量滴定板中对表1中所述的硫醇进行测定，注意到GSH、Cys和AET的检测限小于2.0 pmol/孔，并且对于这些硫醇中的每一种获得线性校准曲线，直至1000 pmol/孔。灵敏度（由校准曲线的斜率表示）表明，5 b比5a略灵敏。
2923 Angew. Chem. Int. Ed. 2005, 44, 2922–2925 www. angewandte. org 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim with 5 was performed for the thiols described in Table 1 in a 96-well microtitre plate, detection limits of less than 2.0 pmol/well were noted for GSH, Cys, and AET, and a linear calibration curve was obtained for each of these thiols up to 1000 pmol/well. Sensitivity, as expressed by the slope of the calibration curves, suggested that 5b was slightly more sensitive than 5a.