A small angle light scattering device for planar connective tissue microstructural analysis
A small angle light scattering device for planar connective tissue microstructural analysis
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DOI：
10.1007/bf02684845
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发表时间：
1997-07-01
影响因子：
3.8
通讯作者：
Hiester, ED
中科院分区：
文献类型：
--
作者：
Sacks, MS;Smith, DB;Hiester, ED
身体的平面纤维结缔组织由嵌入基质中的胶原蛋白和弹性蛋白纤维的致密细胞外网络组成，因此可以被认为是生物复合材料。因此，纤维结构的量化是了解健康和疾病中平面组织力学的重要一步。我们已经使用小角光散射（SALS）映射几个软膜结缔组织的总纤维方向。然而，这些研究中使用的设备和分析方法需要大量的人工干预，不适合大规模纤维结构映射研究。我们已经开发出一种改进的SALS设备，允许快速数据采集，自动化高空间分辨率的标本定位，和新的分析方法，适合大规模的测绘研究。广泛的验证实验表明，SALS设备可以精确地测量纤维取向高达至少500 μ m的组织厚度，以接近1度的角分辨率和+/-254 μ m的空间分辨率。为了证明新设备的能力，从猪主动脉瓣叶的结构测量。结果表明，新的SALS设备提供了一种准确的方法，快速定量的总纤维结构的平面结缔组织。
The planar fibrous connective tissues of the body are composed of a dense extracellular network of collagen and elas tin fibers embedded in a ground matrix, and thus can be thought of as biocomposites. Thus, the quantification of fiber architecture is an important step in developing an understanding of the mechanics of planar tissues in health and disease. We have used small angle light scattering (SALS) to map the gross fiber orientation of several soft membrane connective tissues. However, the device and analysis methods used in these studies required extensive manual intervention and were unsuitable for large-scale fiber architectural mapping studies. We have developed an improved SALS device that allows for rapid data acquisition, automated high spatial resolution specimen positioning, and new analysis methods suitable for large-scale mapping studies. Extensive validation experiments revealed that the SALS device can accurately measure fiber orientation for up to a tissue thickness of at least 500 mu m to an angular resolution of similar to 1 degrees and a spatial resolution of +/-254 mu m. To demonstrate the new device's capabilities, structural measurements from porcine aortic valve leaflets are presented. Results indicate that the new SALS device provides an accurate method for rapid quantification of the gross fiber structure of planar connective tissues.