A marker-assisted analysis of bolting tendency in sugar beet (Beta vulgaris L)
A marker-assisted analysis of bolting tendency in sugar beet (Beta vulgaris L)
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DOI：
10.1023/a:1002915623657
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发表时间：
1997-01-01
期刊：
影响因子：
1.9
通讯作者：
Shimamoto, Y
中科院分区：
文献类型：
--
作者：
Abe, J;Guan, GP;Shimamoto, Y
甜菜抽苔倾向因育种系和品种而异。为了研究抽苔倾向的遗传基础，在易抽苔和抗抽苔的育种系之间进行了四次杂交。经过 8 周的低温处理后，F-2 的抽苔率在杂交中有所不同，表明抽苔倾向存在复杂的遗传控制。观察到不同的分离比，特别是在来自抽苔 F-1 植物的科和来自其非抽苔同胞的科之间，前科显示出明显高于后者的抽苔百分比。对 Ak1、Gdh2、Idh1、Lap、Mdh1、Pgi2 和 Pgm1 七个同工酶位点的标记辅助分析表明，对抽苔倾向有显着影响的位点位于 Idh1 附近。由于 Idh1 与 B 的年发生性紧密连锁，Idh1 标记的基因似乎与 B' 等效或相似，易于与 B 等位基因（如 Owen 等人报道的）。 （1940）。获得的结果表明B基因座可能不仅在决定生长习性方面发挥重要作用，而且还控制个体甜菜植株不同程度的抽苔倾向。还发现一对相连的 Ak1-Lap 和 Pgi2 会影响抽苔倾向，尽管它们的影响因测试的杂交和家族而异。
Bolting tendency in sugar beet varies among breeding lines and cultivars. Four crosses were made between breeding lines susceptible and resistant to bolting in order to study the genetic basis of bolting tendency. Bolting percentage in F-2, after 8 weeks of low temperature treatment, varied among the crosses, suggesting a complicated genetic control of bolting tendency. Different segregation ratios were observed, in particular, between families derived from the bolting F-1 plants and those from their non-bolting siblings, the former families showing a significantly higher bolting percentage than the latter. A marker-assisted analysis with seven isozyme loci, Ak1, Gdh2, Idh1, Lap, Mdh1, Pgi2 and Pgm1, revealed that a locus with marked effect on bolting tendency was located near Idh1. Because of a close linkage of Idh1 with B for annuality, the gene tagged by Idh1 appeared to be equivalent or similar to B' for easy bolting allelic to B as reported by Owen et al. (1940). The results obtained suggest that the B locus may not only have an important role in determining growth habits but also control various degrees of bolting tendency in individual sugar beet plants. A linked pair, Ak1-Lap, and Pgi2 also were found to affect bolting tendency, although their effects varied depending on the crosses and families tested.