Neovascularization in hyperplastic, metaplastic and potentially preneoplastic lesions of the bronchial mucosa
Neovascularization in hyperplastic, metaplastic and potentially preneoplastic lesions of the bronchial mucosa
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支气管粘膜增生性、化生性和潜在癌前病变中的新生血管形成
DOI：
10.1007/bf00192431
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
K. Müller
中科院分区：
文献类型：
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作者：
A. Fisseler‐Eckhoff;D. Rothstein;K. Müller
血管生成在许多正常和病理过程中都是重要的，包括肿瘤的生长和发展、炎症和伤口愈合。我们调查了支气管粘膜的增生性、化生性和潜在的癌前病变中是否存在新生血管作为肺癌的前期。对86例患者的活检标本进行光镜观察。用福尔马林固定石蜡包埋的无炎症的正常支气管黏膜标本12例，与炎症反应(n=9)、基底细胞和杯状细胞增生(n=24)、鳞状细胞化生(n=9)、鳞状细胞化生伴不同程度不典型增生(n=11)、微乳头状瘤病(n=9)和13例原位癌标本进行比较。新生血管的分级用微血管密度来评估，微血管密度是用第VIII因子相关抗原对内皮细胞进行免疫组织化学染色获得的，并由自动图像分析系统确定。在固有膜基底膜下0.4 mm×100 mm的视野中，选择新生血管最多的区域进行微血管计数。以微血管计数、血管最小直径和最大直径为形态变量。黏膜炎症组微血管数显著增加，为33条/0.6mm2(正常支气管炎组为20条/0.6mm2)。炎症支气管炎标本的微血管直径(切面)增加到11.3×10−4mm2(正常支气管壁为9.0 4×10−4mm2)。在鳞状细胞化生(33条/0.6mm2)、鳞状细胞化生伴不同程度不典型增生(50条/0.6mm2)和原位癌(61条/0.6mm2)病例中，微血管计数增加。随着异型增生程度的增加，基底膜区附近的新生血管增多。同时可见内皮细胞上皮间发芽。因此，在潜在的癌前病变中发现了定性和定量的差异。
Angiogenesis is important in a large number of normal and pathological processes including tumour growth and development, inflammation and in wound healing. We investigated whether neovascularization exists in hyperplastic, metaplastic and potentially preneoplastic lesions of the bronchial mucosa as prestages for lung cancer. Biopsy specimens from 86 patients were investigated light microscopically. Formalin-fixed and paraffin-embedded specimens of regular bronchial mucosa including epithelium, basement membrane zone and tunica propria (n=12) without inflammation were compared with specimens with inflammatory reaction (n=9), basal cell- and goblet cell hyperplasia (n=24), squamous cell metaplasia (n=9), squamous cell metaplasia with different degrees of dysplasia (n=11), specimens of micropapillomatosis (n=9) and 13 cases with carcinoma in situ. The grade of neovascularization was assessed by the microvessel density, which was obtained by an immunohistochemical staining of endothelial cells using factor VIII-related antigen and determined by an automatic image-analysing-system. Microvessels were counted in selected areas of highest neovascularization on a×100 field 0.4 mm underneath the basement membrane zone in the tunica propria. Microvessel count, minimal and maximal diameter of the vessels were chosen as morphological variables. A significantly increased microvessel count with 33 vessels/0.6 mm2was found in specimens with inflammation of the tunica mucosa (regular bronchial mucosa: 20 vessels/0.6 mm2). Microvessel diameter (surface of cut section) increased in specimens of bronchial mucosa with inflammation to 11.3×10−4mm2(regular bronchial mucosa: 9.04×10−4mm2). Microvessel count increased in cases of squamous cell metaplasia (33 vessels/0.6 mm2) squamous cell metaplasia with different degrees of dysplasia (50 vessels/0.6 mm2) and carcinoma in situ with 61 vessels/0.6 mm2. With increasing dysplasia, increasing neovascularization was found in close vicinity to the basement membrane zone. Simultaneously, interepithelial sprouts of endothelial cells were seen. Qualitative and quantitative differences were thus found in potentially preneoplastic lesions.