A puzzle assembly strategy for fabrication of large engineered cartilage tissue constructs.
A puzzle assembly strategy for fabrication of large engineered cartilage tissue constructs.
复制标题
用于制造大型工程软骨组织结构的拼图组装策略。
DOI：
10.1016/j.jbiomech.2016.01.023
复制
发表时间：
2016
影响因子：
2.4
通讯作者：
Hung,ClarkT
中科院分区：
文献类型：
--
作者：
Nover,AdamB;Jones,BrianK;Yu,WilliamT;Donovan,DanielS;Podolnick,JeremyD;Cook,JamesL;Ateshian,GerardA;Hung,ClarkT
由于组织生长受到营养扩散的限制，大型关节软骨组织构造的工程仍然是一个挑战。在这里，研究了一种新颖的策略，通过组装单独培养的、连锁的、较小的拼图形状的亚基来生成大型构建体。与较大的结构（杨氏模量大约高 4 倍）相比，这些结构可以一致地设计出更理想的机械和生化特性。开发了一种失效测试技术来评估构建体的生理功能，将其作为单独的亚基培养 28 天，然后组装并再培养 21-35 天。组装好的拼图结构在失效之前能够承受大变形（40-50% 压缩应变）。它们承受生理负荷的能力可以通过增加亚基强度和组装培养时间来增强。利用裸鼠模型来展示体内组装拼图的生物相容性和融合性。总体而言，该技术提供了一种新颖、有效的方法来扩大工程组织的规模，并且可以与其他技术相结合和/或应用于其他组织的工程化。未来的研究将旨在优化该系统，以设计和集成强大的子单元来填补大缺陷。
Engineering of large articular cartilage tissue constructs remains a challenge as tissue growth is limited by nutrient diffusion. Here, a novel strategy is investigated, generating large constructs through the assembly of individually cultured, interlocking, smaller puzzle-shaped subunits. These constructs can be engineered consistently with more desirable mechanical and biochemical properties than larger constructs (~4-fold greater Young׳s modulus). A failure testing technique was developed to evaluate the physiologic functionality of constructs, which were cultured as individual subunits for 28 days, then assembled and cultured for an additional 21–35 days. Assembled puzzle constructs withstood large deformations (40–50% compressive strain) prior to failure. Their ability to withstand physiologic loads may be enhanced by increases in subunit strength and assembled culture time. A nude mouse model was utilized to show biocompatibility and fusion of assembled puzzle pieces in vivo. Overall, the technique offers a novel, effective approach to scaling up engineered tissues and may be combined with other techniques and/or applied to the engineering of other tissues. Future studies will aim to optimize this system in an effort to engineer and integrate robust subunits to fill large defects.