Static biofilm cultures of Gram-positive pathogens grown in a microtiter format used for anti-biofilm drug discovery.
Static biofilm cultures of Gram-positive pathogens grown in a microtiter format used for anti-biofilm drug discovery.
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DOI：
10.1002/0471141755.ph13a08s50
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发表时间：
2010-09-01
影响因子：
--
通讯作者：
Opperman, Timothy J
中科院分区：
文献类型：
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作者：
Kwasny, Steven M;Opperman, Timothy J
An in vitro assay is presented for culturing staphylococcal biofilms and biofilms of nonmotile Gram-positive bacteria under static conditions in microtiter assay plates, and for the quantification of biofilm growth, using a simple staining procedure that measures amounts of bacterial cells and extracellular matrix. This basic assay can be adapted readily to study several aspects of biofilm formation, for high-throughput screening to identify small molecule inhibitors of biofilm formation or biofilm-defective mutants, and for quantifying the anti-biofilm activity of biofilm inhibitors.
