Differences in ectomycorrhizal community assembly between native and exotic pines are reflected in their enzymatic functional capacities
Differences in ectomycorrhizal community assembly between native and exotic pines are reflected in their enzymatic functional capacities
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DOI：
10.1007/s11104-019-04355-9
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发表时间：
2019-11
期刊：
影响因子：
4.9
通讯作者：
C. Ning;W. Xiang;G. Mueller;L. Egerton-Warburton;Wende Yan;Shuguang Liu
中科院分区：
文献类型：
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作者：
C. Ning;W. Xiang;G. Mueller;L. Egerton-Warburton;Wende Yan;Shuguang Liu
背景与目的将外来树种引入自然范围外造林可能会改变当地的外生菌根真菌群落。外来树木与招募的当地ECM真菌而不是本地树木形成的潜在功能后果尚不清楚。本研究考察了(a)本土马尾松（Pinus massoniana）和外来湿地松（Pinus elliottii）在苗期ECM真菌群落的组成和胞外酶功能是否存在差异；(b)酶功能的差异是如何与寄主植物的生长模式联系起来的。方法将本地（马尾松）和外来（湿地松）幼苗种植在每个研究点采集的土芯中。在生长3个月时，收集幼苗的根尖，利用高通量测序分析ECM真菌群落组成，并利用单根尖分析分析与氮、磷和碳获取相关的酶的功能。结果马尾松上的secm真菌对氮（n-乙酰氨基葡萄糖苷酶，280 ~ 300%）、磷（酸性磷酸酶，105 ~ 152%）和纤维素（β-葡萄糖苷酶，204 ~ 235%；纤维素生物水解酶，142 ~ 255%）复合降解酶的活性高于湿地松上的真菌。这些差异归因于某些ECM真菌分类群（如rhizopogonspp）的宿主特异性表现。信息理论模型选择表明，马尾松的植物营养状况与根瘤菌的酶贡献相关，而湿地松则依赖于来自多个ECM真菌类群的不同酶调色板。结论真菌的同源性对ECM真菌群落组成和特定真菌类群的胞外酶功能有显著影响，并对恢复生态系统的寄主建立和养分循环过程有反馈作用。因此，在选择退化土地恢复树种时，造林树种的来源应是一个重要的考虑因素。
Background and AimsIntroducing exotic tree species for afforestation out of their natural range may alter the local ectomycorrhizal (ECM) fungal communities. The potential functional consequences shaped by exotic trees with recruited local ECM fungi rather than native trees remain unclear. This study examined (a) whether the composition and extracellular enzyme function of ECM fungal communities differed between native masson pine (Pinus massoniana) and exotic slash pine (Pinus elliottii) during seedling establishment; and (b) how differences in enzyme functioning were linked to the growth pattern of the host plants.MethodsNative (masson) and exotic (slash) pine seedlings were planted into soil cores collected from each study site. At three months growth, root tips were collected from seedlings and assayed for ECM fungal community composition using high-throughput sequencing, and functioning using single root tip assays for enzymes associated with N, P and C acquisition.ResultsECM fungi on masson pines showed higher activities of nitrogen- (N-acetylglucosaminidase, 280–300%), phosphorus- (acid phosphatase, 105–152%), and cellulose (β-glucosidase, 204–235%; cellobiohydrolase, 142–255%) compound degrading enzymes compared to those on slash pines. Those differences was attributed to the host-specific performance of certain ECM fungal taxa, such asRhizopogonspp. Information theory model selection showed that plant nutrient status in masson pines was correlated with the enzymatic contribution ofRhizopogon spp.,whereas slash pines depended on a diverse enzyme palette from multiple ECM fungal taxa.ConclusionsHost identity strongly influenced ECM fungal community composition and extracellular enzymatic functions of specific ECM fungal taxa, which could feedback to host establishment and nutrient cycling processes of restored ecosystem. Therefore, the origin of afforestation tree species should be an important factor when selecting tree species for restoration of degraded lands.