An aptamer-based protein biochip
An aptamer-based protein biochip
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DOI：
10.1021/ac0483421
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发表时间：
2005-06-01
影响因子：
7.4
通讯作者：
Lindner, P
中科院分区：
文献类型：
--
作者：
Stadtherr, K;Wolf, H;Lindner, P
建立了一种基于核酸适体的蛋白质检测芯片。使用包括人IgE作为分析物和对IgE或抗IgE抗体特异性的单链DNA适体作为芯片上的固定配体的模型系统，我们可以证明适体在特异性和灵敏度方面分别等同于或上级于抗体。在10 ng/mL IgE的最低浓度下，可以清楚地证明载玻片上基于适体的分析物检测。此外，我们还成功地利用基于适体的生物芯片系统对复杂蛋白质样品进行了特异性分析物识别。使用特异于人凝血酶的DNA适体作为额外的模型受体/配体系统，可以证明在单个载玻片上进行双蛋白检测。总之，我们可以显示核酸适体作为生物芯片上的低分子量受体用于敏感和特异性蛋白质检测的适用性，代表未来蛋白质组学的创新工具。
The establishment of an aptamer-based biochip for protein detection is described. Using a model system comprising human IgE as the analyte and single-stranded DNA aptamers specific for IgE or anti-IgE antibodies as immobilized ligands on chips, we could demonstrate that aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. Aptamer-based analyte detection on glass slides could clearly be demonstrated at minimum concentrations of 10 ng/mL IgE. In addition, we successfully showed specific analyte recognition in complex protein samples by the aptamerbased biochip system. Using DNA aptamers specific for human thrombin as an additional model receptor/ligand system, dual protein detection on a single slide could be proven. In conclusion, we could show the suitability of nucleic acid aptamers as low molecular weight receptors on biochips for sensitive and specific protein detection, representing an innovative tool for future proteomics.