Occurrence and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus pseudintermedius in an Academic Veterinary Hospital
Occurrence and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus pseudintermedius in an Academic Veterinary Hospital
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DOI：
10.1128/aem.02780-09
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发表时间：
2010-08-01
影响因子：
4.4
通讯作者：
Tamura, Yutaka
中科院分区：
文献类型：
--
作者：
Ishihara, Kanako;Shimokubo, Natsumi;Tamura, Yutaka
最近，耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林假中间葡萄球菌（MRSP）已越来越多地从兽医和伴侣动物中分离出来。为了防止MRSA和MRSP的传播，我们评估了兽医学院中每种细菌的发生和分子特征。MRSA和MRSP分离自兽医医院附属兽医、工作人员和兽医学生的鼻样本。使用逐步逻辑回归，我们确定了与MRSA携带相关的两个因素：（i）与确定的动物MRSA病例接触（比值比[OR]，6.9; 95%置信区间[95% CI]，2.2至21.6）和（ii）是雇员（OR，6.2; 95% CI，2.0至19.4）。从兽医医院附属个体和犬患者中获得的大多数MRSA分离株携带spa型t002和II型葡萄球菌盒式染色体mec（SCCmec），与日本医院获得的MRSA分离株相似。携带spa型t008和IV型SCCmec的MRSA分离株在三个不同的采样时间从一名兽医和犬患者中获得。MRSA携带者也可能是动物中MRSA感染的来源。大多数MRSP分离株（85.2%）携带混合SCCmec II-III型，几乎所有剩余的MRSP分离株（11.1%）携带SCCmec V型。MRSA和MRSP也从兽医院收集的环境样本中分离（分别为5.1%和6.4%）。在兽医医疗实践中，应采取一定的消毒措施预防医院感染，并应采取MRSA和MRSP感染控制策略。
Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of each in a veterinary college. MRSA and MRSP were isolated from nasal samples from veterinarians, staff members, and veterinary students affiliated with a veterinary hospital. Using stepwise logistic regression, we identified two factors associated with MRSA carriage: (i) contact with an identified animal MRSA case (odds ratio [OR], 6.9; 95% confidence interval [95% CI], 2.2 to 21.6) and (ii) being an employee (OR, 6.2; 95% CI, 2.0 to 19.4). The majority of MRSA isolates obtained from individuals affiliated with the veterinary hospital and dog patients harbored spa type t002 and a type II staphylococcal cassette chromosome mec (SCCmec), similar to the hospital-acquired MRSA isolates in Japan. MRSA isolates harboring spa type t008 and a type IV SCCmec were obtained from one veterinarian on three different sampling occasions and also from dog patients. MRSA carriers can also be a source of MRSA infection in animals. The majority of MRSP isolates (85.2%) carried hybrid SCCmec type II-III, and almost all the remaining MRSP isolates (11.1%) carried SCCmec type V. MRSA and MRSP were also isolated from environmental samples collected from the veterinary hospital (5.1% and 6.4%, respectively). The application of certain disinfection procedures is important for the prevention of nosocomial infection, and MRSA and MRSP infection control strategies should be adopted in veterinary medical practice.