Functional bionetworks from nanoliter water droplets
Functional bionetworks from nanoliter water droplets
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DOI：
10.1021/ja072292a
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发表时间：
2007-07-11
影响因子：
15
通讯作者：
Bayley, Hagan
中科院分区：
文献类型：
--
作者：
Holden, Matthew A.;Needham, David;Bayley, Hagan
我们通过将水滴浸入油/脂混合物中来形成网络。当液滴连接在一起时，它们周围的脂单分子层在界面上结合形成坚固的脂双层。各种蛋白质通道和孔可以结合到液滴-界面双层(DIB)中，在液滴中嵌入电极的电位允许通过界面驱动离子电流并进行测量。通过将液滴连接成线性或分支几何形状，可以创建功能生物网络。虽然相邻液滴之间的界面只由单一的脂双层组成，但网络结构是长期和坚固的。事实上，单个液滴可以通过手术从网络中移除，并被新的液滴取代，而不会破坏相邻的DIBS。液滴网络可以通过使用离子梯度或光驱动的质子泵细菌视紫红质的内部“生物电池”来供电。除了作为偶联原细胞，这些液滴还可以用作超稳定双层记录的装置，大大减少了电解液体积，这将使它们能够用于快速筛选应用。
We form networks from aqueous droplets by submerging them in an oil/lipid mixture. When the droplets are joined together, the lipid monolayers surrounding them combine at the interface to form a robust lipid bilayer. Various protein channels and pores can incorporate into the droplet-interface bilayer (DIB), and the application of a potential with electrodes embedded within the droplets allows ionic currents to be driven across the interface and measured. By joining droplets in linear or branched geometries, functional bionetworks can be created. Although the interfaces between neighboring droplets comprise only single lipid bilayers, the structures of the networks are long-lived and robust. Indeed, a single droplet can be "surgically" excised from a network and replaced with a new droplet without rupturing adjacent DIBs. Networks of droplets can be powered with internal "biobatteries" that use ion gradients or the light-driven proton pump bacteriorhodopsin. Besides their interest as coupled protocells, the droplets can be used as devices for ultrastable bilayer recording with greatly reduced electrolyte volume, which will permit their use in rapid screening applications.