Four-angle saturation transfer (FAST) method for measuring creatine kinase reaction rates in vivo
Four-angle saturation transfer (FAST) method for measuring creatine kinase reaction rates in vivo
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DOI：
10.1002/mrm.10130
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发表时间：
2002-05-01
影响因子：
3.3
通讯作者：
Weiss, RG
中科院分区：
文献类型：
--
作者：
Bottomley, PA;Ouwerkerk, R;Weiss, RG
本文介绍了一种快速测定两位化学交换动力学反应速率的新方法。该方法使用饱和转移磁共振波谱(MRS)，在部分饱和、高信噪比(SNR)条件下仅采集四个波谱。在两次收购中，交换物种中的一个是饱和的；另外两个采用了控制饱和。每对采集被施加两个不同的翻转角，并由此计算平衡磁化、弛豫时间和反应速率。这种四角饱和转移(FAST)方法通过修改为两态化学交换的Bloch方程进行了理论验证。对为骨骼肌和心肌建模的肌酸激酶(CK)代谢，评估了由于交换影响而导致的速率测量中的潜在误差，并发现
A new fast method of measuring kinetic reaction rates for two-site chemical exchange is described. The method employs saturation transfer magnetic resonance spectroscopy (MRS) and acquisition of only four spectra under partially saturated, high signal-to-noise ratio (SNR) conditions. In two acquisitions one of the exchanging species is saturated; the other two employ a control saturation. Each pair of acquisitions is applied with two different flip angles, and the equilibrium magnetization, relaxation times, and reaction rates are calculated therefrom. This four-angle saturation transfer (FAST) method is validated theoretically using the Bloch equations modified for two-state chemical exchange. Potential errors in the rate measurements due to the effects of exchange are evaluated for creatine kinase (CK) metabolism modeled for skeletal and heart muscle, and are found to be