Effect of washing on motility and acrosome morphology of frozen-thawed goat spermatozoa.
Effect of washing on motility and acrosome morphology of frozen-thawed goat spermatozoa.
复制标题
洗涤对冻融山羊精子活力和顶体形态的影响。
DOI：
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发表时间：
1985
影响因子：
1
通讯作者：
R. S. Ott
中科院分区：
文献类型：
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作者：
M. Memon;K. Bretzlaff;R. S. Ott
使用人工阴道收集来自4只巴克斯的精液，并将其合并并分成6等份。将三个等分试样用林格氏溶液洗涤两次，每次15分钟，并在洗涤之间通过以950 X g离心除去流体。用脱脂乳-甘油、乳糖-蛋黄-甘油或三（羟甲基）氨基甲烷-柠檬酸-蛋黄-甘油对所有6个等分试样（3个洗涤的和3个未洗涤的）进行扩展，并在吸管中冷冻至-196 ℃。然后将精液解冻并在37 ℃下保持8小时。在解冻后0、2、4、6和8小时，估计精子活力的百分比，并确定正常顶体（NA）的百分比。实验重复7次。数据表明洗涤对运动性有显著的积极作用（P = 0.0009），但延长剂没有作用（P = 0.5347）。洗涤精液中NA的百分比也显著较高（P <0.0001）。在三氨基甲烷-柠檬酸-蛋黄-甘油中延伸的精子比在乳糖-蛋黄-甘油中延伸的精子具有更多的NA。精子活力和顶体形态也被压抑的存在下，精浆的牛奶扩展，其中不含蛋黄。从山羊精液中去除精浆有利于保存冷冻后精子的完整性，无论使用何种稀释剂。
Semen from 4 bucks was collected using an artificial vagina and was pooled and divided into 6 aliquots. Three aliquots were washed twice, 15 minutes each time, with Ringer's solution, and the fluid was removed by centrifugation at 950 X g between washes. All 6 aliquots (3 washed and 3 unwashed) were extended with skim milk-glycerol, lactose-egg yolk-glycerol, or tris (hydroxymethyl) aminomethane-citric acid-egg yolk-glycerol and were frozen in straws to -196 C. The semen was then thawed and kept at 37 C for 8 hours. Percentage of sperm motility was estimated, and the percentage of normal acrosomes (NA) was determined at 0, 2, 4, 6, and 8 hours after thawing. The experiment was repeated 7 times. The data indicated a significant positive effect (P = 0.0009) of washing on motility, but no effect (P = 0.5347) of extender. There was also a significantly higher percentage of NA in washed semen (P less than 0.0001). Sperm extended in tris aminomethane-citric acid-egg yolk-glycerol had more NA than those extended in lactose-egg yolk-glycerol. Sperm motility and acrosome morphology were depressed also in the presence of seminal plasma for the milk extender, which did not contain egg yolk. Removal of seminal plasma from goat semen was beneficial in preserving the integrity of the spermatozoa after freezing, regardless of the extender used.