The mannose receptor functions as a high capacity and broad specificity antigen receptor in human dendritic cells
The mannose receptor functions as a high capacity and broad specificity antigen receptor in human dendritic cells
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DOI：
10.1002/eji.1830270941
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发表时间：
1997-09-01
影响因子：
5.4
通讯作者：
Pieters, J
中科院分区：
文献类型：
--
作者：
Engering, AJ;Cella, M;Pieters, J
与B淋巴细胞不同，树突状细胞必须能够有效地内化多种抗原，以加工并加载到主要组织相容性复合体（MHC）II类分子上。在这里，我们的特点是在树突状细胞中的甘露糖受体途径，并表明，甘露糖受体介导的抗原摄取的结果在一个类似的100倍更有效的介绍给T细胞，通过液相内化的抗原相比。免疫细胞化学以及亚细胞分级显示甘露糖受体和MHC II类分子在不同的亚细胞区室的本地化。因此，甘露糖受体在各种糖基化抗原的快速内化和浓缩中起作用，所述糖基化抗原变得可用于加工和呈递。这可能有助于树突状细胞产生针对感染因子的初级T细胞应答的独特能力。
Dendritic cells, in contrast to B lymphocytes, must be able to efficiently internalize a diverse array of antigens for processing and loading onto major histocompatibility complex (MHC) class II molecules. Here we characterize the mannose receptor pathway in dendritic cells and show that mannose receptor-mediated uptake of antigens results in a similar to 100-fold more efficient presentation to T cells, as compared to antigens internalized via fluid phase. Immunocytochemistry as well as subcellular fractionation revealed the localization of the mannose receptor and MHC class II molecules in distinct subcellular compartments. The mannose receptor thus functions in rapid internalization and concentration of a variety of glycosylated antigens that become available for processing and presentation. This may contribute to the unique capacity of dendritic cells to generate primary T cell responses against infectious agents.