Identification of C-type lectin-domain proteins (CTLDPs) in silkworm Bombyx mori
Identification of C-type lectin-domain proteins (CTLDPs) in silkworm Bombyx mori
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家蚕 C 型凝集素结构域蛋白 (CTLDP) 的鉴定
DOI：
10.1016/j.dci.2015.07.005
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发表时间：
2015-12-01
影响因子：
2.9
通讯作者：
Yu, Xiao-Qiang
中科院分区：
文献类型：
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作者：
Rao, Xiang-Jun;Shahzad, Toufeeq;Yu, Xiao-Qiang
C型凝集素（CTL）代表了一个大家族的蛋白质，其通常可以以钙依赖性方式结合碳水化合物部分。CTL在免疫系统中介导细胞粘附和病原体识别中起重要作用。本研究共鉴定了23个家蚕CTL基因。CTL结构域蛋白（CTLDPs）根据其糖识别结构域（CRD）的数目和结构域的结构分为三类。这些包括12种CTL-S（单CRD），6种免疫素（双CRD）和5种CTL-X（具有其他结构域的CRD）。我们研究了它们的系统发育特征，分析了保守残基，预测了三级结构，并检测了组织表达谱和免疫诱导。通过生物信息学分析，我们初步鉴定了10种分泌型和2种胞质型CTL-S; 4种分泌型和2种胞质型免疫凝集素; 1种分泌型、1种胞质型和3种跨膜型CTL-X。大多数B。桑CTLDPs与鳞翅目、双翅目、鞘翅目和膜翅目物种的直系同源物形成单系群。B的免疫凝集素。mori和Manducasexta由共同的祖先基因进化而来，可能是由于CTL-S祖先基因的基因复制事件。同源模建揭示了B.家蚕CTL结构域类似于具有可变环区的人的CTL结构域。我们检查了表达谱。CTLDP基因在幼稚和免疫刺激组织中的表达。CTLDP基因的表达和诱导与组织和微生物有关。本研究的基因鉴定、序列比较、系统发育分析、同源性建模和表达分析为B的进一步研究奠定了良好的基础。和比较基因组学。(C)2015爱思唯尔有限公司版权所有。
C-type lectins (CTLs) represent a large family of proteins that can bind carbohydrate moieties normally in a calcium-dependent manner. CTLs play important roles in mediating cell adhesion and the recognition of pathogens in the immune system. In the present study, we have identified 23 CTL genes in domestic silkworm Bombyx mon. CTL-domain proteins (CTLDPs) are classified into three groups based on the number of carbohydrate-recognition domains (CRDs) and the domain architectures. These include twelve CTL-S (Single-CRD), six immulectins (Dual-CRD) and five CTL-X (CRD with other domains). We studied their phylogenetic features, analyzed the conserved residues, predicted tertiary structures, and examined the tissue expression profile and immune inducibility. Through bioinformatics analysis, we have putatively identified ten secretory and two cytoplasmic CTL-S; four secretory and two cytoplasmic immulectins; one secretory, one cytoplasmic and three transmembrane forms of CTL-X. Most B. mori CTLDPs form monophyletic groups with orthologs from Lepidoptera, Diptera, Coleoptera and Hymenoptera species. Immulectins of B. mori and Manduca sexta evolved from common ancestor genes perhaps due to gene duplication events of CTL-S ancestor genes. Homology modeling revealed that the overall structures of B. mori CTL domains are analogous to those of humans with a variable loop region. We examined the expression profile. of CTLDP genes in naive and immune-stimulated tissues. The expression and induction of CTLDP genes were related to the tissues and microorganisms. Together, our gene identification, sequence comparison, phylogenetic analysis, homology modeling and expression analysis laid a good foundation for the further studies of B. mori CTLDPs and comparative genomics. (C) 2015 Elsevier Ltd. All rights reserved.