NUCLEI FROM RAT LIVER - ISOLATION METHOD THAT COMBINES PURITY WITH HIGH YIELD
NUCLEI FROM RAT LIVER - ISOLATION METHOD THAT COMBINES PURITY WITH HIGH YIELD
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DOI：
10.1126/science.154.3757.1662
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发表时间：
1966-01-01
期刊：
影响因子：
56.9
通讯作者：
POTTER, VR
中科院分区：
文献类型：
--
作者：
BLOBEL, G;POTTER, VR
本文描述了一种从大鼠肝脏匀浆中几乎定量分离干净细胞核的方法。它是对使用浓缩蔗糖溶液而不是柠檬酸或洗涤剂的方法的改进，因此可以进一步定量分离细胞质成分。
A procedure is described that gives almost quantitative separation of clean nuclei from a homogenate of rat liver. It is a modification of methods that use concentrated sucrose solutions rather than citric acid or detergents and, therefore, permits further quantitative fractionation of cytoplasmic components.