RATTLESNAKE AND SCORPION ANTIVENOMS FROM THE EGG-YOLKS OF IMMUNIZED HENS
RATTLESNAKE AND SCORPION ANTIVENOMS FROM THE EGG-YOLKS OF IMMUNIZED HENS
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DOI：
10.1038/nbt1090-934
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发表时间：
1990-10-01
期刊：
影响因子：
--
通讯作者：
CARROLL, SB
中科院分区：
文献类型：
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作者：
THALLEY, BS;CARROLL, SB
用于治疗有毒咬伤和蜇伤的抗蛇毒血清通常来自马血清。因此，它们含有马免疫球蛋白，经常引起补体介导的副作用，和其他蛋白质，可以引起血清病，偶尔，过敏性休克。在这里，我们描述了一种新的，禽源的抗蛇毒血清，排除这些并发症，和一个有效的和温和的手段，用于制备抗蛇毒血清组成的毒液特异性抗体。我们证明，从用响尾蛇毒和五纹拟尾蝎毒免疫的产蛋鸡的蛋黄中纯化的抗蛇毒血清中和了这些毒液在体内的致死作用。从鸡蛋中纯化的抗蛇毒血清可能比目前的马抗蛇毒血清更安全，生产更经济。
Antivenoms used to treat poisonous bites and stings are usually derived from horse sera. Consequently, they contain horse immunoglobulins, which frequently cause complement mediated side effects, and other proteins that can cause serum sickness and, occasionally, anaphylactic shock. Here we describe a new, avian source of antivenoms that precludes these complications, and an efficient and gentle means for preparing antivenoms composed solely of venom-specific antibodies. We demonstrate that antivenoms purified from the egg yolks of laying hens immunized withCrotalus atroxrattlesnake venom andLeiurus quinquestriatus hebraeusscorpion venom neutralize the lethal effects of these venomsin vivo. Antivenoms purified from chicken eggs may be phar-maceutically safer and more economical to produce than current horse antivenoms.