Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures
Effect of biotic elicitors on the accumulation of bilobalide and ginkgolides in Ginkgo biloba cell cultures
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DOI：
10.1016/j.jbiotec.2008.09.007
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发表时间：
2009-01-01
影响因子：
4.1
通讯作者：
Choi, Myung-Suk
中科院分区：
文献类型：
--
作者：
Kang, Seung-Mi;Min, Ji-Yun;Choi, Myung-Suk
研究了生物诱导子对银杏细胞悬浮培养生产银杏内酯和银杏内酯的影响。用白色念珠菌和金黄色葡萄球菌作为诱导剂处理细胞培养物，增加了白果内酯（BB）、银杏内酯A（GA）和银杏内酯B（GB）的量，并有轻微的生长抑制。天然细菌诱导子对次级代谢产物在细胞和培养基中的积累比高压灭菌更有效。然而，细胞暴露于激发子的时间强烈影响BB、GA和GB的产生。本研究表明，生物诱导子可以直接或间接地调节BB、GA和GB的产生。这些结果还描述了确定生物诱导子对白果内酯次生代谢影响的最佳条件的建立。皇冠版权所有（c）2008年出版的爱思唯尔B.V.保留所有权利。
The effect of biotic elicitors on the production of bilobalide and ginkgolides in Ginkgo biloba cell suspension cultures was studied. The treatment of cell cultures with Candida albicans and Staphylococcus aureus as elicitors increased the amounts of bilobalide (BB), ginkgolide A (GA) and ginkgolide B (GB), with slight growth inhibition. The native bacterial elicitor was more effective for secondary metabolite accumulations both in cells and culture medium than autoclaved. However, exposure times of the cells to the elicitors strongly influenced the production of BB, GA and GB. This study suggests that biotic elicitors can regulate the production of BB, GA and GB either directly or indirectly. These results also describe the establishment of optimum conditions that determine the effects of biotic elicitors on secondary metabolism of bilobalides. Crown Copyright (c) 2008 Published by Elsevier B.V. All rights reserved.